Kevin H Zhao, 39Ann Arbor, MI

2023000, Jan 5, 2023

Jan 23, 2020

17/425261

- Cambridge MA, US
Kevin Tianmeng Zhao - Cambridge MA, US

The Broad Institute, Inc. - Cambridge MA
President and Fellows of Harvard College - Cambridge MA

C12N 9/22
C07K 14/575
C07K 14/47
C12N 9/78
A61K 9/51

Provided herein are compositions, systems, and methods for delivering an effector protein into a cell. The present disclosure, in some aspects, provide novel proteins delivering an effector protein into a cell. The novel proteins are supernegatively charged proteins derived from highly anionic proteins identified from the proteome (e.g., human proteome). The novel protein tags can be associated (e.g., covalently or nocovalently) with the protein to be delivered to facilitate delivery of the effector protein into a cell.

2020019, Jun 18, 2020

Aug 30, 2018

16/643376

- Cambridge MA, US
Kevin Tianmeng Zhao - Cambridge MA, US
Yongjoo Kim - Cambridge MA, US

President and Fellows of Harvard College - Cambridge MA

C12N 9/22
C12N 15/62
C12N 9/78
C07K 14/47

Some aspects of this disclosure provide strategies, systems, reagents, methods, and kits that are useful for the targeted editing of nucleic acids, including editing a single site within the genome of a cell or subject, e.g., within the human genome. In some embodiments, fusion proteins comprise a Gam protein, a napDNAbp, and a cytidine deaminase. In some embodiments, the fusion proteins further comprise a UGI domain. In some embodiments, methods for targeted nucleic acid editing are provided. In some embodiments, reagents and kits for the generation of targeted nucleic acid editing proteins, e.g., fusion proteins of a Gam protein, a cytidine deaminase and nucleic acid editing proteins or domains, are provided.