Bin Liu resumes & CV records
Resumes
Bin Liu - New Haven, CT
Work:
Yale University Nov 2013 to 2000
Associate Research Scientist Howard Hughes Medical Institute, Yale University Nov 2008 to Nov 2013
Research Associate The Scripps Research Institute Sep 2006 to Nov 2008
Research Associate School of Biological Sciences, Nanyang Technological University - Singapore Sep 2003 to Apr 2006
Teaching assistant in practical and tutorials the Second Hospital of Ezhou, Hubei Province, China Aug 1997 to Aug 1999
A Medical Doctor
Associate Research Scientist Howard Hughes Medical Institute, Yale University Nov 2008 to Nov 2013
Research Associate The Scripps Research Institute Sep 2006 to Nov 2008
Research Associate School of Biological Sciences, Nanyang Technological University - Singapore Sep 2003 to Apr 2006
Teaching assistant in practical and tutorials the Second Hospital of Ezhou, Hubei Province, China Aug 1997 to Aug 1999
A Medical Doctor
Education:
School of Biological Science, Nanyang Technological University - Singapore 2002 to 2006
Ph.D in Structural Biology Wuhan University, China 1999 to 2002
MS in Medical Degree Wuhan University, China 1992 to 1997
BA in Medical Degree
Ph.D in Structural Biology Wuhan University, China 1999 to 2002
MS in Medical Degree Wuhan University, China 1992 to 1997
BA in Medical Degree
Skills:
(1) Well experienced in molecular cloning techniques including plasmid construct design, site-directed mutagenesis, etc. (2) Well experienced in protein expression (E. coli), protein refolding, protein purification using FPLC [affinity (Histag, GST and MBP recombinant protein purification), size-exclusion (superdex75 and 200), ion-exchange (MonoQ MonoS DEAE Heparin), and hydrophobic chromatography] and protein characterization techniques including SDS and native PAGE, UV-visible spectroscopy, Particle size analysis using light scattering, CD spectroscopy, Electrospray ionization mass spectrometry (ESI-MS), and Western Blot. (3) Experienced in peptide synthesis, HPLC purification, Maldi-tof Mass spectrometry, and N-terminal protein sequencing. (4) Extensive experience with protein crystals including macromolecular complex, membrane protein crystal, crystallization screening and optimization including membrane protein crystal dehydration and all kinds of seeding techniques (microseeding, macroseeding and streak seeding), cryotechniques, and single crystal spectrometry. (5) Extensive experience with data collection using both in-house X-ray diffractometer (Rigaku MicroMax-007 with R-axis IV++ detector) and synchrotron beam lines (Swiss Light Source, Stanford Synchrotron Radiation Laboratory including remote access, APS, NSLS, and ALS), data processing (HKL2000, IMOSFLM, CCP4i), and use of UNIX/LINUX-base software for data processing. (6) Extensive experience with various software and programs in Protein Crystallography: molecular replacement (MOLREP and PHASER), MAD, SAD and MIR using heavy atom derivatives (BNP, SOLVE, PHENIX, SHARP and CCP4i). (7) Extensive experience with structure refinement (Refmac5, CNS), structure validation and deposition, and use of relative software including COOT, PyMol, O, Moleman2, etc. (8) Supervised the work of multiple undergraduate research assistants at NTU, including training in laboratory safety, basic biochemical techniques, protein purification and crystallization. (9) Assisted in grant proposal writing at NTU and Scripps; manuscript preparation at NTU, Scripps and Yale.