Charles Rodman Cantor, 73306 Chestnut Ave, Swarthmore, PA 19081

6368813, Apr 9, 2002

Mar 23, 2000

09/381430

Gabriel O. Reznik - Boston MA
Takeshi Sano - Needham MA
Sandor Vajda - Medfield MA
Cassandra Smith - Boston MA
Charles Cantor - Del Mar CA

The Trustees of Boston University - Boston MA

G01N 3353

435 75, 435 71, 435 691, 530350, 530300, 536 237, 536 231

Compounds and methods are described for producing streptavidin mutants with changed affinities. In particular, modifications to the sequence of the natural streptavidin gene is described to create amino acid substitutions resulting in greater affinity for biotin substitutes than for biotin.

6436635, Aug 20, 2002

Mar 12, 1996

08/614151

Charles R. Cantor - Boston MA
Cassandra L. Smith - Boston MA

Boston University - Boston MA
Sequenom, Inc. - San Diego CA

C12Q 168

435 6, 536 231

This invention relates to methods for detecting and sequencing of target double-stranded nucleic acid sequences, to nucleic acid probes and arrays of probes useful in these methods, and to kits and systems which contain these probes. Useful methods involve hybridizing the nucleic acids or nucleic acids which represent complementary or homologous sequences of the target to an array of nucleic acid probes. These probe comprise a single-stranded portion, an optional double-stranded portion and a variable sequence within the single-stranded portion. The molecular weights of the hybridized nucleic acids of the set can be determined by mass spectroscopy, and the sequence of the target determined from the molecular weights of the fragments. Nucleic acids whose sequences can be determined include nucleic acids in biological samples such as patient biopsies and environmental samples. Probes may be fixed to a solid support such as a hybridization chip to facilitate automated determination of molecular weights and identification of the target sequence.

6485913, Nov 26, 2002

Oct 2, 2000

09/678620

Thomas Becker - Lineberg, DE
Charles Cantor - Boston MA

Sequenom, Inc. - San Diego CA

C12Q 168

435 6, 436177, 159 1, 159 471, 422 64, 422 65, 422 66, 422 67

Open systems for performing submicroliter reactions are provided. The systems can include a support for performing the reaction; a liquid dispensing system for dispensing a submicroliter amount of a liquid to a site on or in the support; a temperature controlling device for regulating the temperature of the support; and an interface for controlling the amount of liquid dispensed from the liquid dispensing system are provided. Methods using the systems are also provided.

6818394, Nov 16, 2004

Jan 4, 2000

09/297575

Charles R. Cantor - Boston MA

Sequenom, Inc. - San Diego CA

C12Q 168

435 6, 435 89, 435 911, 435174, 435177, 436501

Processes and kits for immobilizing a high density of nucleic acids on an insoluble surface, which are particularly useful for mass spectrometric detection of nucleic acids, are disclosed. Arrays containing the immobilized nucleic acids and use of the immobilized nucleic acids in a variety of solid phase nucleic acid chemistry applications, including nucleic acid synthesis (chemical and enzymatic), hybridization and/or extension, and sequencing, are provided. Serial and parallel dispensing tools that can deliver defined volumes of fluid to generate multi-element arrays of sample material on a substrate surface are further provided. Tools provided herein can include an assembly of vesicle elements, or pins, wherein each of the pins can include a narrow interior chamber suitable for holding nanoliter volumes of fluid. Methods for dispensing tools that can be employed to generate multi-element arrays of sample material on a substrate surface are also provided. The tool can dispense a spot of fluid to a substrate surface by spraying the fluid from the pin, contacting the substrate surface or forming a drop that touches against the substrate surface.

6991903, Jan 31, 2006

Apr 30, 2002

10/136829

Charles R. Cantor - Boston MA, US
Hubert Köster - Concord MA, US
Cassandra L. Smith - Boston MA, US

Sequenom, Inc. - San Diego CA
The Trustees of Boston University - Boston MA

C12Q 1/68
C12P 19/34

435 6, 435 911, 435 912

Methods for detecting and sequencing of target double-stranded nucleic acid molecules, nucleic acid probes and arrays of probes useful in these methods, and kits and systems that contain these probes are provided. The methods involve hybridizing the nucleic acids or nucleic acids which represent complementary or homologous sequences of the target to an array of nucleic acid probes. These probes include a single-stranded portion, an optional double-stranded portion and a variable sequence within the single-stranded portion. The molecular weights of the hybridized nucleic acids of the set can be determined by mass spectroscopy, and the sequence of the target determined from the molecular weights of the fragments.

7232688, Jun 19, 2007

Jul 30, 1999

09/364774

Daniel P. Little - Boston MA, US
Charles R. Cantor - Boston MA, US
Hubert Köster - La Jolla CA, US

Sequenom, Inc. - San Diego CA

G01N 24/00
G01N 1/10
B01D 59/44

436173, 436180, 250287, 250425

Methods for dispensing tools that can be employed to generate multi-element arrays of sample material on a substrate surface. The resulting substrates are also provided. The substrates surfaces can be flat or geometrically altered to include wells of receiving material. The tool can dispense a spot of fluid to a substrate surface by spraying the fluid from the pin, contacting the substrate surface or forming a drop that touches against the substrate surface. The tool can form an array of sample material by dispensing sample material in a series of steps, while moving the pin to different locations above the substrate surface to form the sample array. The prepared sample arrays are passed to a plate assembly that disposes the sample arrays for analysis by mass spectrometry. To this end, a mass spectrometer is provided that generates a set of spectra signals that are indicative of the composition of the sample material under analysis.

7319003, Jan 15, 2008

Feb 24, 1998

09/030571

Charles R. Cantor - Boston MA, US
Cassandra L. Smith - Boston MA, US
Takeshi Sano - Waltham MA, US

The Trustees of Boston University - Boston MA

C12Q 1/68
C12N 1/36
C07H 21/04

435 6, 435174, 4352831, 4352872, 536 231, 536 243

This invention is directed to methods and reagents useful for sequencing nucleic acid targets utilizing sequencing by hybridization technology comprising probes, arrays of probes and methods whereby sequence information is obtained rapidly and efficiently in discrete packages. That information can be used for the detection, identification, purification and complete or partial sequencing of a particular target nucleic acid. When coupled with a ligation step, these methods can be performed under a single set of hybridization conditions. The invention also relates to the replication of probe arrays and methods for making and replicating arrays of probes which are useful for the large scale manufacture of diagnostic aids used to screen biological samples for specific target sequences. Arrays created using PCR technology may comprise probes with 5′- and/or 3′-overhangs.

2002002, Feb 21, 2002

Jun 24, 1998

09/104100

CHANDRAN R. SABANAYAGAM - ALLSTON MA, US
CHARLES R. CANTOR - BOSTON MA, US
CASSANDRA L. SMITH - BOSTON MA, US

C12Q001/00
C12Q001/68
G01N033/53
G01N033/543

435/004000, 435/006000, 435/007100, 435/007500, 438/001000

The coating of silicon surfaces is described, including but not limited to coating silicon surfaces with biomolecules such as biotin and avidin. Silicon surfaces are made with a monolayer of the protein streptavidin and these surfaces are then used as supports for reactions, including but not limited to nucleic acid hybridization.