David S Clague, 641230 Scenic Way, Los Osos, CA 93402

7504265, Mar 17, 2009

Jun 5, 2006

11/447754

David S. Clague - Livermore CA, US
Elizabeth K. Wheeler - Livermore CA, US
Abraham P. Lee - Irvine CA, US

Lawrence Livermore National Security, LLC - Livermore CA

G01N 33/543

436518, 436532, 436534, 436800, 436805, 435 71, 4352831, 4352871, 4352887, 422 8205, 422 8208, 422213, 422215

A flow cytometer includes a flow cell for detecting the sample, an oil phase in the flow cell, a water phase in the flow cell, an oil-water interface between the oil phase and the water phase, a detector for detecting the sample at the oil-water interface, and a hydrophobic unit operatively connected to the sample. The hydrophobic unit is attached to the sample. The sample and the hydrophobic unit are placed in an oil and water combination. The sample is detected at the interface between the oil phase and the water phase.

7711491, May 4, 2010

May 5, 2004

10/840779

Jennifer A. Vandersall - Livermore CA, US
Shea N. Gardner - San Leandro CA, US
David S. Clague - Livermore CA, US

Lawrence Livermore National Security, LLC - Livermore CA

G01N 33/48
C12Q 1/68
C12N 15/11

702 19, 702 20, 435 6, 536 231, 536 241

A computational method and computer-based system of modeling DNA synthesis for the design and interpretation of PCR amplification, parallel DNA synthesis, and microarray chip analysis. The method and system include modules that address the bioinformatics, kinetics, and thermodynamics of DNA amplification and synthesis. Specifically, the steps of DNA selection, as well as the kinetics and thermodynamics of DNA hybridization and extensions, are addressed, which enable the optimization of the processing and the prediction of the products as a function of DNA sequence, mixing protocol, time, temperature and concentration of species.

7871799, Jan 18, 2011

Dec 3, 2003

10/727779

Shea N. Gardner - San Leandro CA, US
Allen T. Christian - Tracy CA, US
Jennifer A. Young - Berkeley CA, US
David S. Clague - Livermore CA, US

Lawrence Livermore National Security, LLC - Livermore CA

C12P 19/34
C12Q 1/68

435 912, 435 6

A method of fabricating a DNA molecule of user-defined sequence. The method comprises the steps of preselecting a multiplicity of DNA sequence segments that will comprise the DNA molecule of user-defined sequence, separating the DNA sequence segments temporally, and combining the multiplicity of DNA sequence segments with at least one polymerase enzyme wherein the multiplicity of DNA sequence segments join to produce the DNA molecule of user-defined sequence. Sequence segments may be of length n, where n is an even or odd integer. In one embodiment the length of desired hybridizing overlap is specified by the user and the sequences and the protocol for combining them are guided by computational (bioinformatics) predictions. In one embodiment sequence segments are combined from multiple reading frames to span the same region of a sequence, so that multiple desired hybridizations may occur with different overlap lengths. In one embodiment starting sequence fragments are of different lengths, n, n+1, n+2, etc.

8470537, Jun 25, 2013

Dec 3, 2010

12/959789

Shea N. Gardner - San Leandro CA, US
Allen T. Christian - Tracy CA, US
Jennifer A. Young - Berkeley CA, US
David S. Clague - Livermore CA, US

Lawrence Livermore National Security, LLC - Livermore CA

C12Q 1/68
C12P 19/34

435 612, 435 912

A method of preselecting a multiplicity of DNA sequence segments that will comprise the DNA molecule of user-defined sequence, separating the DNA sequence segments temporally, and combining the multiplicity of DNA sequence segments with at least one polymerase enzyme wherein the multiplicity of DNA sequence segments join to produce the DNA molecule of user-defined sequence. Sequence segments may be of length n, where n is an odd integer. In one embodiment the length of desired hybridizing overlap is specified by the user and the sequences and the protocol for combining them are guided by computational (bioinformatics) predictions. In one embodiment sequence segments are combined from multiple reading frames to span the same region of a sequence, so that multiple desired hybridizations may occur with different overlap lengths.

8584506, Nov 19, 2013

May 16, 2006

11/435495

James V. Candy - Danville CA, US
David S. Clague - Livermore CA, US
Christopher L. Lee - Oakland CA, US
Robert E. Rudd - Livermore CA, US
Alan K. Burnham - Livermore CA, US
Joseph W. Tringe - Walnut Creek CA, US

lawrence Livermore National Security, LLC. - Livermore CA

G01N 9/00
G01N 31/00

73 2321, 702 27, 702 32

A method of using physics-based signal processing algorithms for micromachined cantilever arrays. The methods utilize deflection of a micromachined cantilever that represents the chemical, biological, or physical element being detected. One embodiment of the method comprises the steps of modeling the deflection of the micromachined cantilever producing a deflection model, sensing the deflection of the micromachined cantilever and producing a signal representing the deflection, and comparing the signal representing the deflection with the deflection model.

2005027, Dec 8, 2005

Jun 7, 2004

10/862998

David Clague - Livermore CA, US
Elizabeth Wheeler - Livermore CA, US
Abraham Lee - Irvine CA, US

G01N033/53
G01N033/567
G01N033/542
C12M001/34

435007200, 435287200

A flow cytometer includes a flow cell for detecting the sample, an oil phase in the flow cell, a water phase in the flow cell, an oil-water interface between the oil phase and the water phase, a detector for detecting the sample at the oil-water interface, and a hydrophobic unit operatively connected to the sample. The hydrophobic unit is attached to the sample. The sample and the hydrophobic unit are placed in an oil and water combination. The sample is detected at the interface between the oil phase and the water phase.

2009019, Aug 6, 2009

Feb 1, 2008

12/024762

George M. Dougherty - Albany CA, US
John M. Dzenitis - Danville CA, US
Robin R. Miles - Danville CA, US
David S. Clague - San Luis Obispo CA, US

B01D 57/02
B01D 17/06
B01D 17/00
B01D 17/12

204547, 204450, 204600, 204643, 210748, 210767, 210243

Systems and methods for separating particles and/or toxins from a sample fluid. A method according to one embodiment comprises simultaneously passing a sample fluid and a buffer fluid through a chamber such that a fluidic interface is formed between the sample fluid and the buffer fluid as the fluids pass through the chambers the sample fluid having particles of interest therein; applying a force to the fluids for urging the particles of interest to pass through the interface into the buffer fluid; and substantially separating the buffer fluid from the sample fluid.