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Dean E Hillman Deceased203 Round Hill Rd, East Hills, NY 11577

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203 Round Hill Rd, Roslyn Heights, NY 11577    516-7239180   

36 Old Lyme Rd, Chappaqua, NY 10514    914-2383451   

East Lansing, MI   

1104 Lucia Ave, Fairmont, MN 56031    507-2355159   

620 Kelly Pl, Onalaska, WI 54650   

New Hyde Park, NY   

New York, NY   

Nassau, NY   

Roslyn Hts, NY   

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Dean Hillman

Location:
United States

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Us Patents

Methods For Direct Biomolecule Identification By Matrix-Assisted Laser Desorption Ionization (Maldi) Mass Spectrometry

US Patent:
8525104, Sep 3, 2013
Filed:
Apr 27, 2010
Appl. No.:
12/799576
Inventors:
Paul Pevsner - New York NY, US
Frederick Naftolin - Woodbridge CT, US
Douglas C. Miller - Belle Mead NJ, US
Dean Hillman - New Hyde Park NY, US
Brian K. Stall - Bedford NH, US
Steven M. Wishnies - Columbia MD, US
Assignee:
New York University - New York NY
International Classification:
B01D 59/44
H01J 49/00
H01J 49/40
US Classification:
250281, 250288, 250282
Abstract:
The present invention relates to the use of post source decay (PSD) or collision induced dissociation (CID) direct tissue (DT) MALDI-TOF or DT-MALDI-TOF-TOF mass spectrographic identification of biological molecules in a tissue or cellular sample without the need for further protein extraction. This method provides for studying cells or tissues by direct tissue MALDI (DT-MALDI), thereby substituting in situ protein release for further protein extraction. Mass/intensity data was processed with Mascot software interrogation of the NCBI database. These results are proof of principle that DT-MALDI, combined with bioinformatics, can directly identify proteins in cells and tissues from their mass spectra.

Methods For Direct Biomolecule Identification By Matrix-Assisted Laser Desorption Ionization (Maldi) Mass Spectrometry

US Patent:
2007011, May 24, 2007
Filed:
Sep 29, 2006
Appl. No.:
11/541380
Inventors:
Paul Pevsner - New York NY, US
Frederick Naftolin - Woodbridge CT, US
Douglas Miller - Belle Mead NJ, US
Dean Hillman - New Hyde Park NY, US
Brian Stall - Bedford NH, US
Steven Wishnies - Columbia MD, US
International Classification:
B01D 59/44
US Classification:
250282000
Abstract:
The present invention relates to the use of post source decay (PSD) or collision induced dissociation (CID) direct tissue (DT) MALDI-TOF or DT-MALDI-TOF-TOF mass spectrographic identification of biological molecules in a tissue or cellular sample without the need for further protein extraction. This method provides for studying cells or tissues by direct tissue MALDI (DT-MALDI), thereby substituting in situ protein release for further protein extraction. Mass/intensity data was processed with Mascot software interrogation of the NCBI database. These results are proof of principle that DT-MALDI, combined with bioinformatics, can directly identify proteins in cells and tissues from their mass spectra.

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