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Dennis M Disorbo, 7612 Presidential Dr, Wilmington, MA 01887

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12 Presidential Dr, Wilmington, MA 01887    978-6578375   

Olean, NY   

Princeton, MA   

Hudson, NH   

Buffalo, NY   

Phoenix, AZ   

Lake Placid, NY   

Philadelphia, PA   

12 Presidential Dr, Wilmington, MA 01887   

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Biotechnology

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Dennis Disorbo

Location:
Greater Boston Area
Industry:
Biotechnology

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Us Patents

Media Concentrate Technology

US Patent:
5681748, Oct 28, 1997
Filed:
May 19, 1995
Appl. No.:
8/444678
Inventors:
Dennis M. DiSorbo - Buffalo NY
David Jayme - Grand Island NY
Assignee:
Life Technologies, Inc. - Rockville MD
International Classification:
C12N 500
C12N 104
US Classification:
435404
Abstract:
The present invention relates to a method of subgrouping media formulations into stable, compatible components that can then be solubilized at high concentrations (10. times. to 100. times. ). Concentrated solutions of the ingredient containing subgroups can be used to prepare media formulations. Concentrated culture media formulations (2-10. times. ) or 1. times. cell culture media can be prepared according to the present invention by mixing a sufficient amount of the concentrated subgroup solutions with a sufficient amount of a diluent (water, buffer, etc. ). The present invention further relates to stabilization of glutamine by complexing or mixing glutamine with divalent metal cations in solution.

Media Concentrate Technology

US Patent:
5474931, Dec 12, 1995
Filed:
Sep 21, 1993
Appl. No.:
8/124508
Inventors:
Dennis M. DiSorbo - Buffalo NY
David W. Jayme - Grand Island NY
Assignee:
Life Technologies, Inc. - Gaithersburg MD
International Classification:
C12N 500
C12N 104
US Classification:
43524031
Abstract:
The present invention relates to a method of subgrouping media formulations into stable, compatible components that can then be solubilized at high concentrations (10. times. to 100. times. ). Concentrated solutions of the ingredient containing subgroups can be used to prepare media formulations. Concentrated culture media formulations (2-10. times. ) or 1. times. cell culture media can be prepared according to the present invention by mixing a sufficient amount of the concentrated subgroup solutions with a sufficient amount of a diluent (water, buffer, etc. ). The present invention further relates to stabilization of glutamine by complexing or mixing glutamine with divalent metal cations in solution.

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