Dong Qin, 64Mercer Island, WA

2004025, Dec 23, 2004

Feb 27, 2004

10/788884

Paul Yager - Seattle WA,
Mark Holl - Seattle WA,
Darrel Bell - Van Couver WA,
James Brody - Pasadena CA,
Catherine Cabrera - Seattle WA,
Andrew Kamholz - Seattle WA,
Katerina Macounova - Seattle WA,
Dong Qin - Kenmore WA,

University of Washington - Seattle WA

B01D059/50
G01N027/26
G01L001/20

204/450000, 204/600000

Devices and methods are provided for separation of particles of a first selected electrophoretic mobility or isoelectric point from a fluid comprising particles of at least one other selected electrophoretic mobility or isoelectric point. The devices comprise a microchannel comprising an inlet for introducing the fluid into the microchannel; electrodes to either side of the microchannel for applying a selected voltage to produce an electrical field across the microchannel orthogonal to the length of the microchannel; and outlets in said microchannel placed to receive outlet portions of the fluid containing enhanced concentrations of each type of particle. The devices may be used for particle detection, quantification, separation, mixing, dilution and concentration. Electrophoretic tags may be used to provide particles with altered electrophoretic mobilities and/or isoelectric points. Interior particles of cells or organisms may be released, separated and detected by these devices and methods. The devices and methods may be used to separate particles such as proteins and microorganisms from biological fluids such as blood, or to separate and detect airborne contaminants such as bacterial warfare agents from air. The devices and methods may be included in devices including other separation and detection methods.

2012013, Jun 7, 2012

Nov 13, 2007

11/939292

Paul Yager - Seattle WA,
Mark Holl - Seattle WA,
Darrel J. Bell - Vancouver WA,
James Brody - Pasadena CA,
Catherine R. Cabrera - Seattle WA,
Andrew E. Kamholz - Seattle WA,
Katerina Macounova - Seattle WA,
Dong Qin - Kenmore WA,

University of Washington - Seattle WA

B01D 57/02

204548, 204644

Devices and methods are provided for separation of particles of a first selected electrophoretic mobility or isoelectric point from a fluid comprising particles of at least one other selected electrophoretic mobility or isoelectric point. The devices comprise a microchannel; electrodes to either side of the microchannel for applying a selected voltage to produce an electrical field across the microchannel orthogonal to the length of the microchannel; and outlets in said microchannel placed to receive outlet portions of the fluid containing enhanced concentrations of each type of particle. The devices may be used for particle detection, quantification, separation, mixing, dilution and concentration; to release, separate and detect interior particles of cells or organisms, and to separate particles such as proteins and microorganisms from biological fluids such as blood; or to separate and detect airborne contaminants such as bacterial warfare agents from air.

6304364, Oct 16, 2001

Jun 11, 1997

8/873191

Dong Qin - Seattle WA
Younan Xia - Seattle WA
George M. Whitesides - Newton MA

President & Fellows of Harvard College - Cambridge MA

G02B 2600

359291

Electromagnetic radiation valves are provided. In one system, a transparent, elastomeric article is provided having a contoured surface including protrusions and intervening indentations. The contoured surface is placed adjacent a transparent plate and a void pattern defined by indentations of the contoured surface is filled with an opaque fluid. The protrusions decrease in cross-sectional area in the direction in which they extend away from the bulk of the elastomeric article, thus compression of the elastomeric protrusions against the transparent plate results in an increase in surface area in elastomeric protrusions against the transparent plate due to deformation of the protrusions, and the opaque fluid is displaced from the voids. Increased transmission of the electromagnetic radiation results. In another system, a similar elastomeric article includes an array of corner cubes that are totally internally reflective of the electromagnetic radiation.