Donna L Paddock, 72115 Elm St UNIT 13A, Hatfield, MA 01038
Donna Paddock Phones & Addresses
115 Elm St UNIT 17A, Hatfield, MA 01038 413-2476224
85 Plain Rd, Sunderland, MA 01375 413-6657026
South Deerfield, MA
Niantic, CT
Kalamazoo, MI
Mentions for Donna L Paddock
Publications & IP owners
Us Patents
Method For Refolding Enzymes
US Patent:
7732183, Jun 8, 2010
Filed:
Feb 7, 2007
Appl. No.:
11/703493
Inventors:
Alfredo Tomasselli - Kalamazoo MI, US
Robert Heinrikson - Plainwell MI, US
Donna Paddock - Kalamazoo MI, US
Ana Mildner - Kalamazoo MI, US
Thomas Emmons - Portage MI, US
Robert Heinrikson - Plainwell MI, US
Donna Paddock - Kalamazoo MI, US
Ana Mildner - Kalamazoo MI, US
Thomas Emmons - Portage MI, US
Assignee:
Elan Pharmaceuticals, Inc. - South San Francisco CA
International Classification:
C12N 9/50
US Classification:
435219, 435226
Abstract:
The invention provides methods for efficient recombinant expression, refolding, and purification of Beta-site APP cleaving enzyme (BACE) polypeptides. In various aspects, the method includes the steps of expressing a recombinant construct in bacteria, dissolving inclusion bodies with a denaturant at high pH in the presence of a reducing agent, diluting the solubilized BACE polypeptide in an aqueous solution at a temperature of about 1 C. to 15 C. , and incubating the diluted sample at a temperature of about 4 C. to 15 C. until the recombinant BACE polypeptide folds into an active enzyme.
Method For Refolding Enzymes
US Patent:
7186539, Mar 6, 2007
Filed:
Aug 29, 2002
Appl. No.:
10/230677
Inventors:
Alfredo Tomasselli - Kalamazoo MI, US
Robert Heinrikson - Plainwell MI, US
Donna Paddock - Kalamazoo MI, US
Ana Mildner - Kalamazoo MI, US
Thomas Emmons - Portage MI, US
Robert Heinrikson - Plainwell MI, US
Donna Paddock - Kalamazoo MI, US
Ana Mildner - Kalamazoo MI, US
Thomas Emmons - Portage MI, US
Assignee:
Pharmacia & UpJohn Company - Kalamazoo MI
International Classification:
C12N 9/50
US Classification:
435219, 435226
Abstract:
The invention provides methods for efficient recombinant expression, refolding, and purification of Beta-site APP cleaving enzyme (BACE) polypeptides. In various aspects, the method includes the steps of expressing a recombinant construct in bacteria, dissolving inclusion bodies with a denaturant at high pH in the presence of a reducing agent, diluting the solubilized BACE polypeptide in an aqueous solution at a temperature of about 1 C. to 15 C. , and incubating the diluted sample at a temperature of about 4 C. to 15 C. until the recombinant BACE polypeptide folds into an active enzyme.
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