Flavio G Silva, 522817 38Th Ave UNIT 4C, Long Island City, NY 11101

8460905, Jun 11, 2013

Sep 11, 2007

11/853339

Christopher L. G. Dayton - Mount Kisco NY, US
Erin Marie Rosswurm - Columbus IN, US
Flavio da Silva Galhardo - Manteno IL, US

Bunge Oils, Inc. - St. Louis MO

C12P 7/64
C12P 7/00
C12N 9/20
A61K 36/48
C12P 1/00
C12N 9/00
C12N 9/14
C02F 1/00
C11B 13/00
C11B 3/02

435134, 435 41, 435183, 435195, 435198, 210632, 554175, 424757

A method for degumming an oil composition comprises the steps of (a) providing an oil composition containing a quantity of phospholipids, (b) contacting said oil composition simultaneously with one or more phospholipase A enzymes and one or more phospholipase C enzymes, under conditions sufficient for the enzymes to react with the phospholipids to create phospholipid reaction products, and (c) separating the phospholipids reaction products from the oil composition, the remaining oil composition after the separation being a degummed oil composition, whereby during step (b) the reaction of said one or more phospholipase A enzymes proceeds at a faster rate than it would in the absence of said one or more phospholipase C enzymes, and wherein the reaction of step (b) continues for a duration of less than about one hour.

2013001, Jan 10, 2013

Oct 8, 2010

13/502105

Christopher L.G. Dayton - Mount Kisco NY, US
Flavio Da Silva Galhardo - Scarsdale NY, US
Nelson Barton - San Diego CA, US
Tim Hitchman - Carlsbad CA, US
Jonathan Lyon - San Diego CA, US
Eileen O'Donoghue - San Diego CA, US
Mark A. Wall - San Diego CA, US

C11B 3/02
A23D 7/02
C09K 3/00
A23D 7/00
C12P 9/00
C11C 1/08

435131, 435271, 2521823, 426602, 426 33

In alternative embodiments, the invention provides phosphatidylinositol-specific phospholipase C (PI-PLC) enzymes, nucleic acids encoding them, antibodies that bind specifically to them, and methods for making and using them. Industrial methods and products comprising use of these phospholipases are also provided. In certain embodiments, provided herein are methods for hydration of non hydratable phospholipids (NHPs) within a lipid matrix. The methods enable migration of NHPs to an oil-water interface thereby allowing the NHPs to be reacted and/or removed from the lipids. In certain embodiments, provided is a method for removing NHPs, hydratable phospholipids, and lecithins from vegetable oils to produce a degummed oil or fat product that can be used for food production and/or non-food applications. In certain embodiments, provided herein are methods for hydration of NHPs followed by enzymatic treatment and removal of various phospholipids and lecithins. The methods provided herein can be practiced on either crude or water-degummed oils.

2014037, Dec 18, 2014

Aug 19, 2014

14/463557

- White Plains NY, US
Flavio Da Silva Galhardo - Scarsdale NY, US
Nelson Barton - San Diego CA, US
Tim Hitchman - Carlsbad CA, US
Jonathan Lyon - San Diego CA, US
Eileen O'Donoghue - San Diego CA, US
Mark A. Wall - San Diego CA, US

C11B 3/00

554 1, 435134

In alternative embodiments, the invention provides phosphatidylinositol-specific phospholipase C (PI-PLC) enzymes, nucleic acids encoding them, antibodies that bind specifically to them, and methods for making and using them. Industrial methods and products comprising use of these phospholipases are also provided. In certain embodiments, provided herein are methods for hydration of non hydratable phospholipids (NHPs) within a lipid matrix. The methods enable migration of NHPs to an oil-water interface thereby allowing the NHPs to be reacted and/or removed from the lipids. In certain embodiments, provided is a method for removing NHPs, hydratable phospholipids, and lecithins from vegetable oils to produce a degummed oil or fat product that can be used for food production and/or non-food applications. In certain embodiments, provided herein are methods for hydration of NHPs followed by enzymatic treatment and removal of various phospholipids and lecithins. The methods provided herein can be practiced on either crude or water-degummed oils.