Gary Joseph Dahl, 734825 Zenith Ave S, Minneapolis, MN 55410

6562575, May 13, 2003

Jun 26, 2001

09/891661

Gary A. Dahl - Madison WI

Epicentre Technologies Corporation - Madison WI

C12Q 168

435 6, 435 911

Methods and compositions are provided for assaying for a target analyte in a sample by formation of a substrate for a replicase. The target analyte, if present in the sample, is first bound to a reporter probe. The reporter probe comprises a first portion and a second portion. The first portion comprises a polynucleotide that encodes at least part of a sequence for an RNA that is a substrate for replication. The second portion comprises a molecule that has affinity for an analyte. The reporter probe itself is not a substrate for a replicase. However, a replicase substrate is generated by treating reporter probe which is bound to analyte with a composition having nuclease activity in order to release the parts of the first portion, and then the released parts and one or more mononucleotides or oligonucleotides comprising missing parts of the substrate sequence are joined with a composition having ligase activity. Newly formed substrate is replicated with a replicase, and a signal is measured which indicates the presence and quantity of analyte in the sample. The invention also provides methods and compositions for simultaneously assaying for any of multiple analytes in a sample.

7413857, Aug 19, 2008

Nov 21, 2003

10/719168

Gary A. Dahl - Madison WI, US
Jerome J. Jendrisak - Madison WI, US
Agnes J. Radek - Madison WI, US

Epicentre Technologies - Madison WI

C12Q 1/68
C12P 19/34
C07H 21/04

435 6, 435 912, 536 2433

Methods, compositions and kits for amplifying a target sequence by strand displacement replication using strand-displacing primers. The method uses primers that have only ribonucleotides or purine ribonucleotides and at least one 2′-substituted pyrimidine-2′-deoxyribonucleotide.

7659072, Feb 9, 2010

Aug 5, 2008

12/186127

Gary A. Dahl - Madison WI, US
Jerome J. Jendrisak - Madison WI, US
Agnes J. Radek - Madison WI, US

Epicentre Technologies Corporation - Madison WI

C12Q 1/68
C12P 19/34
C07H 21/04

435 6, 435 912, 536 2431, 536 24

Methods, compositions and kits for amplifying a target sequence by strand displacement replication using strand-displacing primers. The method uses primers that have only ribonucleotides or purine ribonucleotides and at least one 2′-substituted pyrimidine-2′-deoxyribonucleotide.

7727744, Jun 1, 2010

Mar 30, 2005

11/093387

Michael J. Fiandt - Cambridge WI, US
Gary A. Dahl - Madison WI, US

Epicentre Technologies Corporation - Madison WI

C12P 21/02
C12P 21/06
C12N 15/64
C12N 15/00

435 701, 435 691, 435 9141, 435455

Methods, compositions and kits are disclosed for obtaining directionally truncated polypeptides by inserting a transposon. Preferably the transposon comprises a selectable marker and an ori, and optionally a promoter, a ribosome binding site and a translation start codon, into a target sequence in vitro or in vivo. Amplification products, varying in length depending on the transposon insertion site, are obtained using one primer that anneals to the target sequence and a second primer that anneals to the transposon. Amplification products are ligated to circular dsDNA, transformed into host cells, and individual colonies, each containing a directionally truncated clone of the target sequence, are obtained by plating on medium for which the selectable marker encodes resistance. Directionally truncated polypeptides encoded by the target sequence are obtained in vivo by inducing an RNAP in the host cells that uses the promoter or, in vitro by cell-free transcription and translation.

8039214, Oct 18, 2011

Jun 30, 2008

12/165324

Gary Dahl - Madison WI, US
Roy Rabindranauth Sooknanan - Beaconsfield, CA

CellScript, Inc. - Madison WI

C12Q 1/68
C12P 19/34

435 611, 435 612, 435 911, 435 912

The present invention relates generally to methods, compositions and kits for synthesizing sense RNA molecules from one or more RNA molecules of interest in a sample. In exemplary embodiments, the methods use a terminal tagging oligoribonucleotide (rTTO) to join a DNA sequence tag to the 3′-termini of first-strand cDNA molecules. The use of an rTTO comprising ribonucleotides results in decreased oligonucleotide-derived background synthesis of RNA in the absence of sample RNA and, surprisingly and unexpectedly, also results in significantly increased yields of sense RNA molecules that exhibit sequences that are substantially identical to those of the RNA molecules of interest in the sample. The sense RNA molecules also have an RNA sequence tag on their 5′-termini that is useful for fixing the lengths of sense RNA molecules that are synthesized in a second or subsequent round.

8137911, Mar 20, 2012

Nov 21, 2003

10/719372

Gary A. Dahl - Madison WI, US
Jerome J. Jendrisak - Madison WI, US
Elena K. Davydova - Chicago IL, US
Svetalana Y. Gerdes - Madison WI, US

CellScript, Inc. - Madison WI

C12Q 1/68
C12P 19/34

435 61, 435 912

The present invention provides methods, compositions and kits for using an RNA polymerase for making transcription products corresponding to a target sequence by obtaining single-stranded DNA transcription substrates that comprise a single-stranded promoter that is operably joined to a single-stranded target sequence. The invention has broad applicability for research, diagnostic and therapeutic applications, such as preparing cDNA corresponding to full-length mRNA, making sense or anti-sense probes, detecting gene- or organism-specific sequences, cloning, cell signaling or making RNA for use in RNAi.

8163491, Apr 24, 2012

Feb 17, 2010

12/707243

Jerome Jendrisak - Madison WI, US
Ramesh Vaidyanathan - Madison WI, US
Gary Dahl - Madison WI, US

Epicentre Technologies Corporation - Madison WI

C12Q 1/68

435 612

The present invention provides novel compositions, kits and methods employing RNA 5′ polyphosphatases, RNA 5′ monophosphatases, capping enzymes, decapping enzymes, nucleic acid pyrophosphatases and RNA ligases, as well as other enzymes, for selective 5′ ligation tagging of desired classes of RNA molecules that differ with respect to particular chemical moieties on their 5′ ends. The 5′tagged RNA molecules can be used for synthesis of tagged first-stand cDNA, double-stranded cDNA, and sense or antisense RNA for a variety of uses.

8309335, Nov 13, 2012

Feb 29, 2012

13/408851

Jerome Jendrisak - Madison WI, US
Ramesh Vaidyanathan - Madison WI, US
Gary Dahl - Madison WI, US

Epicentre Technologies Corporation - Madison WI

C12N 9/12
C12N 9/22
C12N 9/16

435194, 435199

The present invention provides novel compositions, kits and methods employing RNA 5′ polyphosphatases, RNA 5′ monophosphatases, capping enzymes, decapping enzymes, nucleic acid pyrophosphatases and RNA ligases, as well as other enzymes, for selective 5′ ligation tagging of desired classes of RNA molecules that differ with respect to particular chemical moieties on their 5′ ends. The 5′ tagged RNA molecules can be used for synthesis of tagged first-stand cDNA, double-stranded cDNA, and sense or antisense RNA for a variety of uses.