George Izaak Case, 39205 Old Dirt Rd, Tallahassee, FL 32317

5413915, May 9, 1995

Jul 12, 1991

7/729365

George D. Case - Portland OR
Paul J. Bekowies - Staten Island NY

Resource Technologies Group, Inc. - Morgantown WV

C12Q 126
C01N 2100

435 25

A metabolically active biological component having mixed function oxidation activity. e. g. , microsomal P-450 oxidase enzyme complex, is effective in the presence of NADPH and moisture when dispersed in a thin film of a generally neutral hydrophilic film-forming binder. e. g. , gelatin, to convert a broad spectrum of carcinogenic substances, into metabolic intermediates, which can be detected, preferably by a colorimetric indicator present in the binder film or an adjacent binder film and undergoing a visible color change. The film or films can be carried, e. g. , as spots by a suitable support. e. g. , glass or plastic sheet forming a direct-acting self-contained badge-type indicator. The colorimetric indicator is preferably a chemically reduced dye precursor of a polyarylmethane dye or analog thereof, e. g. , pararosaniline or malachite green.

5328847, Jul 12, 1994

Nov 18, 1991

7/794734

George D. Case - Aloha OR
Jennings F. Worley - Durham NC

C12M 134
C12M 140
G01N 3096
C25F 700

435291

A modular biosensor system for chemical or biological agent detection utilizes electrochemical measurement of an ion current across a gate membrane triggered by the reaction of the target agent with a recognition protein conjugated to a channel blocker. The sensor system includes a bioresponse simulator or "biochemical switch" module which contains the recognition protein-channel blocker conjugate, and in which the detection reactions occur, and a transducer module which contains a gate membrane and a measuring electrode, and in which the presence of agent is sensed electrically. In the poised state, ion channels in the gate membrane are blocked by the recognition protein-channel blocker conjugate. Detection reactions remove the recognition protein-channel blocker conjugate from the ion channels, thus eliciting an ion current surge in the gate membrane which subsequently triggers an output alarm. Sufficiently large currents are generated that simple direct current electronics are adequate for the measurements.