Girish H Kulkarni6745 Country Hill Ave, Cedar Rapids, IA 52402

2023005, Feb 16, 2023

Jan 11, 2021

17/791790

- Ann Arbor MI, US
Mark A. ILGEN - Ann Arbor MI, US
Girish KULKARNI - Ann Arbor MI, US
Maung Kyaw KHAING OO - Ann Arbor MI, US

THE REGENTS OF THE UNIVERSITY OF MICHIGAN - Ann Arbor MI
ARBORSENSE INC. - Ann Arbor MI
OPTOFLUIDIC BIOASSAY, LLC - Ann Arbor MI

G01N 33/94
B01L 3/00

An automated microfluidic bioreactor device and methods to rapidly detect drugs of abuse from human sweat are provided. The bioreactor can perform either single-plexed measurements (detecting only one target analyte at a time) or multiplexed measurement (detecting multiple analytes simultaneously). The bioreactor device has a cartridge comprising a capillary array that employs competitive enzyme-linked immunosorbent assay (ELISA) to detect the presence of various drugs or metabolite compounds. For example, four common drugs, methadone, methamphetamine, amphetamine, and tetrahydrocannabinol, were detected rapidly and quantitatively in about 16 minutes with a low sweat sample volume (about 4 μL per analyte) and a large dynamic range (methadone: 0.0016 ng/mL-1 ng/mL; METH: 0.016 ng/mL-25 ng/mL; amphetamine: 0.005 ng/mL-10 ng/mL; THC: 0.02 ng/mL-1000 ng/mL).

2016029, Oct 6, 2016

Dec 4, 2014

15/037809

- Ann Arbor MI, US
Girish KULKARNI - Ann Arbor MI, US
Karthik REDDY - Ann Arbor MI, US
Xudong FAN - Saline MI, US

G01N 27/414
B01D 53/02

An improved sensing method is provided for rapid analyte detection. The method includes: applying an AC excitation signal to the channel region of the transistor; applying an AC drive signal to the transistor; delivering an analyte of interest to a channel region of a transistor; and monitoring a mixing current of the excitation signal and the drive signal through the transistor, where a change in the mixing current is indicative of the concentration of the analyte of interest.