Gregory J Mcclune, 72815 Eden Green Ct, Roswell, GA 30075

5374516, Dec 20, 1994

Feb 24, 1989

7/315086

Richard C. Sutton - Rochester NY
Brent A. Burdick - Rochester NY
Susan J. Danielson - Rochester NY
Harold C. Warren - Rush NY
Brian A. Snyder - Rochester NY
Gregory J. McClune - Rochester NY
Annie L. Wu - Penfield NY

Eastman Kodak Company - Rochester NY

G01N 33545
G01N 33546

435 5

Reagents have been prepared from water-insoluble polymeric particles to which are covalently attached avidin, biotin or an avidin or biotin derivative. The polymeric particles comprise a polymer on at least the outer surface which is derived from at least one ethylenically unsaturated monomer having a reactive activated 2-substituted ethylsulfonyl, vinylsulfonyl or active halogen atom. Covalent attachment of avidin, biotin or an avidin or biotin derivative is effected either directly or indirectly through these reactive groups. The resulting reagent is useful in analytical elements and various analytical methods including agglutination and sandwich assays. The immobilized avidin, biotin or derivative can be used to complex with the corresponding biotin or avidin molecule which may be conjugated to a compound of biological interest.

4828983, May 9, 1989

Jul 10, 1986

6/884329

Gregory J. McClune - Rochester NY

Eastman Kodak Company - Rochester NY

C12Q 128
G01N 3358
G01N 3352
G01N 3353

435 7

Certain phenols and anilines can be used as electron transfer agents to increase the rate of oxidation of leuco dyes by peroxidase. These phenols and anilines can react with hydrogen peroxide in the presence of peroxidase to provide intermediates which have higher oxidation potentials than the slowly oxidized substrates, e. g. the leuco dyes. These phenols and anilines can be used to advantage in both solution and dry assays of various analytes. They are particularly useful for the determination of an immunologically reactive ligand in an immunoassay.

5366864, Nov 22, 1994

Sep 30, 1992

7/953905

Gregory J. McClune - Portage MI
Karen L. Findling - Rochester NY

Eastman Kodak Company - Rochester NY

G01N 3353
G01N 33547
G01N 3376

435 75

A buffered aqueous composition is useful simultaneously as a wash solution and a dye-providing composition in specific binding assays involving enzyme-labeled specific binding reagents. The wash composition includes a dye-providing composition, a buffer and an organic solvent having a certain molecular weight and water-solubility. Another useful composition includes a particulate substrate having avidin attached thereto, and a peroxidase reducing agent. Either composition can be provided in a diagnostic test kit, and can be used to detect a specific binding ligand in assays.

5176999, Jan 5, 1993

Dec 7, 1989

7/447332

Gregory J. McClune - Portage MI
Karen L. Findling - Rochester NY

Eastman Kodak Company - Rochester NY

G01N 3353
G01N 3376

435 75

A buffered aqueous composition is useful simultaneously as a wash solution and a dye-providing composition in specific binding assays involving enzyme-labeled specific binding reagents. The wash composition includes a dye-providing composition, a buffer and an organic solvent having a certain molecular weight and water-solubility. Another useful composition includes a particulate substrate having avidin attached thereto, and a peroxidase reducing agent. Either composition can be provided in a diagnostic test kit, and can be used to detect a specific binding ligand in assays.

5017474, May 21, 1991

Feb 12, 1988

7/155670

Gregory J. McClune - Portage MI
Margaret J. Smith-Lewis - Pittsford NY

Eastman Kodak Company - Rochester NY

G01N 3353
G01N 3348
G01N 33543
G01N 33536

435 75

An aqueous wash solution is buffered to a pH of from about 5 to about 9 and contains at least about 0. 01 weight percent of a compound comprising a dodecyl sulfate anion and an alkali metal or ammonium cation, such as sodium dodecyl sulfate. This wash solution is particularly useful in a method for the determination of an immunological ligand. Particularly, it is useful for washing the immunological complex formed between the ligand and a receptor molecule therefor. Unreacted materials can be readily separated from the complex by the washing, particularly if the separation is carried out using a filtration membrane in a test device. a test kit for ligand determination comprises the wash solution as well as one or more receptors for the ligand, at least one of which is labeled for detection. This kit is particularly useful for measuring human chorionic gonadotropin (hCG) as an early indicator of pregnancy.

5024935, Jun 18, 1991

Dec 18, 1987

7/136166

Gregory J. McClune - Rochester NY
John F. Bishop - Rochester NY

Eastman Kodak Company - Rochester NY

G01N 3353
G01N 33536
C12Q 128
C12N 996

435 71

A dye-providing composition comprises a water-soluble or -dispersible polymer, such as a vinylpyrrolidone polymer, and an imidazole leuco dye capable of providing a dye in the presence of hydrogen peroxide and a peroxidative substance. The weight ratio of polymer to leuco dye is from about 10,000:1 to about 100:1. The dye-providing composition can be included with a peroxidase substrate in a diagnostic test kit. A method for the determination of a ligand can be carried out using a peroxidase labeled-receptor for the ligand and the dye-providing composition described above. The method is particularly useful for the determination of human chorionic gonadotropin (hCG).

4803157, Feb 7, 1989

Jan 31, 1986

6/824752

Michael W. Sundberg - Penfield NY
Bruce Babb - Rochester NY
Gregory McClune - Rochester NY

Eastman Kodak Company - Rochester NY

F12Q 142
C07F 902

435 21

Hydrolyzable substrates for acid and alkaline phosphatases comprise blocked dye moieties which, when cleaved from the substrate during hydrolysis, provide fluorescent dyes having maximum absorptions above about 530 nm and maximum fluorescent emissions at least about 580 nm. The dyes are blocked prior to hydrolysis with a phosphono or thioxophosphono group or a salt thereof. These substrates can be used in analytical determinations of acid or alkaline phosphatase, or in competitive binding reactions to determine immunologically reactive substances.

5185128, Feb 9, 1993

Nov 1, 1990

7/607957

Gregory J. McClune - Portage MI
Margaret J. Smith-Lewis - Pittsford NY

Eastman Kodak Company - Rochester NY

G01N 3353
G01N 3358

422 61

A test kit useful for the determination of an immunological ligand includes a labeled receptor for that ligand, a specific wash solution and a test device. The wash solution is buffered to a pH of from about 5 to about 9 and contains at least 0. 01 weight percent of a compound having a dodecyl sulfate anion and an alkali metal or ammonium cation. One such compound is sodium dodecyl sulfate. The test device has several test wells, and in each well there is a microporous membrane. A receptor for the ligand of interest is immobilized in one test well. The kit is particularly useful for measuring human chorionic gonadotropin as an early indicator of pregnancy.