Mark Jin ZhangOakland, CA

Company: Hoffmann-la roche Sep 2009 Position: Senior research associate

Cellular and functional: Experience with WB • PBMC • lymphocytes • spleenocytes isolation Primary cell culture and general cell culture Luminex • MSD and Aushon based cytokine analysis Multi-color FACS analysis and operation skills (BD LSRII • Fortessa • and Calibur) Fluorescence and confocal microscopy Cell surface receptor quantification (human PBMC and stable cells) Chemotaxis assay using Neuroprobe plates T cell proliferation assay: WST Functional anti-viral assays including cell-cell fusion • pseudoviral-cell fusion Cell-based ELISA • ELISA Ligand-receptor binding assays mAb labeling and purification Molecular Biology: Transfection and stable cell line generation Mutagenesis: domain swap and site-directed mutation Inducible expression system: tet-on • tet-off Pseudo-virus generation Molecular cloning • gene knock-out constructs Nucleic acid purification Northern • southern blot • PCR • RT-PCR • Taqman analysis • competitive qRT-PCR small scale recombinant protein expression and purification quantitative Western blot In vivo: Small animal dissection tissue/organ collection iv • ip • sc • im and oral drug administration Hybridoma injection and ascites collection [] (c) Jun (Mark) Zhang [email protected] Computer skills: Familiar with both PC and Mac MS office suite Specialized programs such as FlowJo • CellQuest • Diva • etc. GraphPad Prism Vector NTI