Heng Wenling W Zhong, 63419 Brookgreen Dr, Chapel Hill, NC 27516

6858777, Feb 22, 2005

Aug 13, 2001

09/928614

Heng Zhong - Chapel Hill NC, US
Eric Boudreau - Durham NC, US
Sabrina Rouse - Clayton NC, US
Erik Dunder - Hillsborough NC, US
Weining Gu - Chapel Hill NC, US
Yin-Fu Chang - Carrboro NC, US

Syngenta Participations AG - Basel

C12N015/82
C12N015/84
A01H004/00
A01H005/00

800294, 800293, 800298, 800308, 800309, 800310, 800322, 800278, 435430, 435469, 435470, 435431

Multiple shoot structures are induced from plant tissues (e. g. , shoot apices or axillary buds on an artificial medium) to produce multiple shoot cultures. These multi-shoot cultures are then transformed by known transformation methods. Plants are subsequently regenerated from the transformed cells. Crops that may be efficiently transformed by this method include plants normally recalcitrant to transformation such as sugar beet, sunflower, soybean, cotton, tobacco, tomato, peanuts, melons, watermelon, squash, and pepper.

2009002, Jan 22, 2009

Jul 19, 2007

11/880107

Heng Zhong - Chapel Hill NC, US
Qiudeng Que - Cary NC, US

Syngenta Participations AG - Basel

A01H 5/00
C12N 5/04

435415, 800312

The present disclosure provides methods for the transformation of soybean cells or tissue and regeneration of the soybean cells or tissue into transformed plants. The disclosed methods utilize an explant prepared from an immature soybean inflorescence, shoot apex, leaf axillary, shoot axillary, or combination thereof which can be induced directly to form shoots that give rise to transgenic plants via organogenesis.

2011032, Dec 29, 2011

Dec 1, 2009

13/139515

Heng Zhong - Chapel Hill NC, US

C12N 15/82

800293, 435468, 435470, 435469, 800278, 800294

Methods for the transformation of sugar cane are provided. The methods comprise utilizing sugar cane immature shoots as the source of plant material for transformation. Segments of the immature shoot are excised and transformed by any suitable transformation methodology. In some embodiments, the segments are cultured in embryogenic culture induction medium prior to transformation. Transformation can be performed via -mediated gene delivery, biolistic transformation, and the like. Transgenic plants are regenerated from plantlets grown under conditions favoring growth of transformed cells while substantially inhibiting growth of non-transformed cells.

2013033, Dec 12, 2013

Jun 10, 2013

13/913937

- Basel, CH
Chunyang Fan - Research Triangle Park NC, US
John Ke - Research Triangle Park NC, US
Heng Zhong - Research Triangle Park NC, US
Doug Russell - Research Triangle Park NC, US

C12Q 1/68

435 612

The invention provides methods and compositions for detecting and/or quantifying vector backbone in a nucleic acid preparation comprising a polynucleotide of interest using amplification assays that amplify a junction located between the polynucleotide of interest and the vector backbone, under conditions whereby amplification can occur, wherein the junction comprises a recognition site for a nuclease, and detecting the absence of an amplification product, whereby the absence of the amplification product indicates low or no vector backbone and/or quantifying the amount of amplification product to determine the amount of vector backbone in the nucleic acid preparation.

5320961, Jun 14, 1994

Nov 16, 1992

7/977253

Heng Zhong - East Lansing MI
Masomeh B. Sticklen - Okemos MI
Chinnathambi Srinivasan - East Lansing MI

Board of Trustees operating Michigan State University - East Lansing MI

C12N 500

43524045

A method for asexual in vitro propagation of corn plants is described. The plant tissue (shoot tips or apices) are differentiated with 2,4-dichlorophenoxy acetic acid (2,4-D) and N. sup. 6 -benzyladenine (BA) and mixtures thereof and then the differentiated tissue is grown in indole-3-butyric acid (IBA) to produce the corn plant which is fertile.

5767368, Jun 16, 1998

Apr 3, 1995

8/415426

Heng Zhong - East Lansing MI
Masomeh B. Sticklen - Okemos MI

Board of Trustees operating Michigan State University - East Lansing MI

C12N 1529
C12N 1582
A01H 400
A01H 500

800205

A method for isolating a cereal plant with foreign DNA by bombarding meristem primordia tissue with particles coated with the foreign DNA in a culture media is described. The foreign DNA in the transformed plants can provide proteins which impart disease and/or insect resistance or other desirable properties.

2022038, Dec 8, 2022

Nov 24, 2020

17/775955

- Basel, CH
Wenjin Yu - Research Triangle Park NC, US
Heng Zhong - Research Triangle Park NC, US
Hua-ping Zhou - Research Triangle Park NC, US

SYNGENTA CROP PROTECTION AG - Basel

C12N 15/82

Conventional gene transformation requires tissue culture, and some elite lines have very low transformation efficiency in tissue culture. The disclosure relates to methods of in planta transformation. In some aspects, an axillary meristem of a plant is wounded and contacted with a transformation agent. The wounded axillary meristem is then regenerated and treated with a selection step, resulting in transformed tissue that can produce transgenic seeds.

2023011, Apr 13, 2023

Aug 26, 2022

17/822537

- Basel, CH
Mark Rose - Research Triangle Park NC, US
Zhongying Chen - Research Triangle Park NC, US
Heng Zhong - Research Triangle Park NC, US
Weining Gu - Research Triangle Park NC, US
Wenling Wang - Research Triangle Park NC, US
Qiudeng Que - Research Triangle Park NC, US
Ailing Zhou - Research Triangle Park NC, US
Mary-Dell Chilton - Research Triangle Park NC, US

SYNGENTA PARTICIPATIONS AG - Basel

C12N 15/82
A01H 6/46

The present invention relates to methods and compositions for targeted insertion of polynucleotide molecules into ideal target sites in the genome of a maize plant. The present invention relates to maize recombinant molecules comprising heterologous sequences and also to methods of integrating a DNA of interest into a target maize genomic locus in a maize genome. The present invention also relates to regenerated maize plants or plant parts comprising the recombinant molecules and/or a DNA of interest.