Lavell R Johnson, 60Lake Point, UT

4009005, Feb 22, 1977

Apr 7, 1975

5/565850

Lavell R. Johnson - Salt Lake City UT

Becton, Dickinson and Company - East Rutherford NJ

G01N 3106
G01N 3316

23253R

Apparatus for radioimmunoassay provides a continuously reusable short cycle analytical system of particular use in biochemical analysis such as the determination of steroids, hormones, polypeptides, viruses, vitamins, drugs, and the like. The apparatus disclosed samples a mixture of an unlabelled antigen sample and a known concentration of labelled antigen, bringing the mixture into contact with a mass of appropriate antibody that has been immobilized thereby to bind at least part of the mixed antigen to the antibody. The amount of bound and/or unbound labelled antigen is detected and counted and, by use of previously prepared standards, the unknown concentration of unlabelled antigen is found. The still immobilized antibody is regenerated for immediate reuse by rinsing it with a selected eluting solution that is capable of breaking the antigen-antibody. Alternate ways of immobilizing the antibody are described.

4059685, Nov 22, 1977

Apr 7, 1975

5/565848

LaVell R. Johnson - Salt Lake City UT

Summa Corporation - Culver City CA

G01N 3100
G01T 100
G21C 1700

424 12

An immobilized immunoadsorbent for use in immunoassay and radioimmunoassay procedures includes, in a preferred form, a mass of superficially porous refractory particles having a water insoluble polymer, such as dextran, bonded thereto. The polymer in turn has bonded to it, by covalent bonds, antibodies capable of binding a specific antigen thereto and stoichiometrically releasing the bound antigen by the use of a rinsing solution which does not adversely affect the binding of the antibody to the polymer. Preferably, each of the porous particles includes an impervious core which has joined thereto sufficient layers of microparticles to form an outer porous coating on the core. Methods of forming such an immunoadsorbent and for using the same and structure for supporting the same are also disclosed.

4238471, Dec 9, 1980

Jul 12, 1978

5/923712

Max G. Reese - Salt Lake City UT
LaVell R. Johnson - Salt Lake City UT

Becton, Dickinson and Company - Paramus NJ

G01N 3316
B01J 122
G21H 502

424 1

Automated assay for qualitative determination of thyroid hormone binding capacity of a serum sample in which serum and T. sub. 3 tracer are caused to flow through a chamber containing a T. sub. 3 binder supported on a solid support. The amount of T. sub. 3 tracer which passes through the chamber, and the amount of T. sub. 3 tracer bound to the binder in the chamber are determined to thereby determine the percent of bound T. sub. 3 tracer. Such percent is then compared with the percent of T. sub. 3 tracer bound in a referenced sample to qualitatively determine the T. sub. 3 binding capacity of the serum sample.

4180383, Dec 25, 1979

May 31, 1977

5/801867

Lavell R. Johnson - Salt Lake City UT

Becton, Dickinson and Company - E. Rutherford NJ

G01N 3316

422 69

An immobilized immunoadsorbent for use in immunoassay and radioimmunoassay procedures includes, in a preferred form, a mass of superficially porous refractory particles having a water insoluble polymer, such as dextran, bonded thereto. The polymer in turn has bonded to it, by covalent bonds, antibodies capable of binding a specific antigen thereto and stoichiometrically releasing the bound antigen by the use of a rinsing solution which does not adversely affect the binding of the antibody to the polymer. Preferably, each of the porous particles includes an impervious core which has joined thereto sufficient layers of microparticles to form an outer porous coating on the core. Methods of forming such an immunoadsorbent and for using the same and structure for supporting the same.

4279885, Jul 21, 1981

May 19, 1978

5/907741

Max G. Reese - Salt Lake City UT
Dennis K. Ransom - Granger UT
LaVell R. Johnson - Salt Lake City UT

Becton Dickinson & Company - Paramus NJ

G01N 3348
G01T 100

424 1

An analyte, such as an antigen, is initially contacted with a receptor (antibody) bound to a solid support, in the absence of tracer, followed by removal of unbound analyte and contacting with tracer. By separately contacting the receptor with analyte and tracer, sensitivity is increased.

4166102, Aug 28, 1979

May 31, 1977

5/802075

Lavell R. Johnson - Salt Lake City UT

Becton, Dickinson and Company - East Rutherford NJ

G01N 3316

424 1

An immobilized immunoadsorbent for use in immunoassay and radioimmunoassay procedures includes, in a preferred form, a mass of superficially porous refractory particles having a water insoluble polymer, such as dextran, bonded thereto. The polymer in turn has bonded to it, by covalent bonds, antibodies capable of binding a specific antigen thereto and stoichiometrically releasing the bound antigen by the use of a rinsing solution which does not adversely affect the binding of the antibody to the polymer. Preferably, each of the porous particles includes an impervious core which has joined thereto sufficient layers of microparticles to form an outer porous coating on the core. Methods of forming such an immunoadsorbent and for using the same and structure for supporting the same are also disclosed.