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James J Cali, 69512 Lawn Ave, Madison, WI 53593

Known as:
James J Call Jim Cali
Related to:
Samuel H Cali 30

James Cali Phones & Addresses

512 Lawn Ave, Verona, WI 53593    608-8488566   

Fitchburg, WI   

Shamokin Dam, PA   

Dallas, TX   

417 8Th St, Selinsgrove, PA 17870    570-3749591   

Johnson City, NY   

Irving, TX   

Dane, WI   

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James Cali resumes & CV records

Resumes

James Cali Photo 35

Research Director

Location:
Madison, WI
Industry:
Biotechnology
Work:
Promega
Research Director
Education:
Ut Southwestern Medical Center 1987 - 1991
Doctorates, Doctor of Philosophy, Molecular Biology University of Illinois at Urbana - Champaign 1973 - 1977
Bachelors, Bachelor of Science, Biology
Skills:
Cell, Assay Development, Life Sciences, Biochemistry, Biotechnology, Adme, High Throughput Screening, Drug Discovery, Molecular Biology, Cell Biology, Lifesciences, Genomics, Protein Chemistry, Signal Transduction, Pcr, Cell Based Assays, Cell Culture, Cell Signaling, Pharmaceutical Industry, Metabolism, Adme Tox, Cytochrome P450 Biochemistry
Interests:
Cell Health and Metabolism
Research Tools Development
Epigenetics
Cell Signaling
James Cali Photo 36

James Cali

James Cali Photo 37

James Cali

James Cali Photo 38

James Cali

Location:
United States
James Cali Photo 39

James Cali

Publications & IP owners

Us Patents

Luciferase-Based Assays

US Patent:
7741067, Jun 22, 2010
Filed:
Dec 23, 2003
Appl. No.:
10/746995
Inventors:
Erika Hawkins - Madison WI, US
James J. Cali - Verona WI, US
Samuel Kin Sang Ho - New Bedford MA, US
Martha A. O'Brien - Madison MA, US
Richard Somberg - Madison WI, US
Robert F. Bulleit - Verona WI, US
Keith V. Wood - Mount Horeb WI, US
Assignee:
Promega Corporation - Madison WI
International Classification:
C12Q 1/66
C12Q 1/00
C12Q 1/08
US Classification:
435 8, 435 4
Abstract:
A method and kit is provided for enhancing the tolerance of an assay reagent to compounds in an assay sample, the assay reagent including a luciferase enzyme. The method includes contacting the luciferase with a tolerance enhancement agent in an amount sufficient to substantially protect luciferase enzyme activity from interference of the compound and minimize interference by at least about 10% relative to an assay not having tolerance enhancement agent.

Luminogenic And Fluorogenic Compounds And Methods To Detect Molecules Or Conditions

US Patent:
7951550, May 31, 2011
Filed:
May 31, 2006
Appl. No.:
11/444145
Inventors:
James J. Cali - Verona WI, US
William Daily - Santa Maria CA, US
Erika Hawkins - Madison WI, US
Dieter Klaubert - Arroyo Grande CA, US
Jianquan Liu - Fremont CA, US
Poncho Meisenheimer - San Luis Obispo CA, US
Michael Scurria - San Luis Obispo CA, US
John W. Shultz - Verona WI, US
James Unch - Arroyo Grande CA, US
Michael P. Valley - Fitchburg WI, US
Keith V. Wood - Mt. Horeb WI, US
Wenhui Zhou - Santa Maria CA, US
Assignee:
Promega Corporation - Madison WI
International Classification:
C12Q 1/66
C12Q 1/26
G01N 33/573
US Classification:
435 8, 435 74, 435 25
Abstract:
A method to detect the presence or amount of at least one molecule in a sample which employs a derivative of luciferin or a derivative of a fluorophore is provided.

Luminescence-Based Methods And Probes For Measuring Cytochrome P450 Activity

US Patent:
8106052, Jan 31, 2012
Filed:
Apr 5, 2010
Appl. No.:
12/754164
Inventors:
James J. Cali - Verona WI, US
Dieter Klaubert - Arroyo Grande CA, US
William Daily - Santa Maria CA, US
Samuel Kin Sang Ho - New Bedford MA, US
Susan Frackman - Madison WI, US
Erika Hawkins - Madison WI, US
Keith V. Wood - Mount Horeb WI, US
Assignee:
Promega Corporation - Madison WI
International Classification:
A61K 31/403
A61K 31/4164
A61K 31/422
A61K 31/437
A61K 31/4985
C07D 413/04
C07D 417/04
C07D 471/04
C07D 487/04
US Classification:
514249, 514299, 514367, 514375, 514393, 548466, 548238, 5483114, 548146, 544236, 546113
Abstract:
The present invention provides methods, compositions, substrates, and kits useful for analyzing the metabolic activity in cells, tissue, and animals and for screening test compounds for their effect on cytochrome P450 activity. In particular, a one-step and two-step methods using luminogenic molecules, e. g. luciferin or coelenterazines, that are cytochrome P450 substrates and that are also bioluminescent enzyme, e. g. , luciferase, pro-substrates are provided. Upon addition of the luciferin derivative or other luminogenic molecule into a P450 reaction, the P450 enzyme metabolizes the molecule into a bioluminescent enzyme substrate, e. g. , luciferin and/or luciferin derivative metabolite, in a P450 reaction. The resulting metabolite(s) serves as a substrate of the bioluminescent enzyme, e. g. , luciferase, in a second light-generating reaction.

Luciferase-Based Assays

US Patent:
8361739, Jan 29, 2013
Filed:
Jun 21, 2010
Appl. No.:
12/819334
Inventors:
Erika Hawkins - Madison WI, US
James J. Cali - Verona WI, US
Samuel Kin Sang Ho - New Bedford MA, US
Martha A. O'Brien - Madison WI, US
Richard Somberg - Madison WI, US
Robert F. Bulleit - Verona WI, US
Keith V. Wood - Mount Horeb WI, US
Assignee:
Promega Corporation - Madison WI
International Classification:
C12Q 1/66
C12Q 1/34
C12Q 1/37
US Classification:
435 8, 435 18, 435 23
Abstract:
A method and kit are provided for enhancing the tolerance of an assay reagent to compounds in an assay sample, the assay reagent including a luciferase enzyme. The method includes contacting the luciferase with a tolerance enhancement agent in an amount sufficient to substantially protect luciferase enzyme activity from interference of the compound and minimize interference by at least about 10% relative to an assay not having tolerance enhancement agent.

Luminogenic And Fluorogenic Compounds And Methods To Detect Molecules Or Conditions

US Patent:
8476450, Jul 2, 2013
Filed:
Jul 3, 2008
Appl. No.:
12/217494
Inventors:
James J. Cali - Verona WI, US
William Daily - Santa Maria CA, US
Erika Hawkins - Pembroke, CA
Dieter Klaubert - Arroyo Grande CA, US
Jianquan Liu - Fremont CA, US
Poncho Meisenheimer - San Lius Obispo CA, US
Michael Scurria - San Luis Obispo CA, US
John Shultz - Verona WI, US
James Unch - Arroyo Grande CA, US
Michael P. Valley - Fitchburg WI, US
Keith V. Wood - Mt. Horeb WI, US
Wenhui Zhou - Santa Maria CA, US
Assignee:
Promega Corporation - Madison WI
International Classification:
A61K 31/428
C07D 417/04
C07D 417/14
US Classification:
548178, 514367
Abstract:
A method to detect the presence or amount of at least one molecule in a sample which employs a derivative of luciferin or a derivative of a fluorophore is provided. Compounds and compositions for carrying out the methods of the invention are also provided.

Bioluminescent Detection Of Cyanohydroxy Benzothiazole Compounds

US Patent:
8551721, Oct 8, 2013
Filed:
Sep 9, 2009
Appl. No.:
12/556505
Inventors:
Jessica Anderson - Monticello WI, US
Poncho Meisenheimer - San Luis Obispo CA, US
John Shultz - Verona WI, US
James J. Cali - Verona WI, US
Dongping Ma - Madison WI, US
Assignee:
Promega Corporation - Madison WI
International Classification:
C12Q 1/66
A61K 38/04
US Classification:
435 8, 435 72, 435 25, 435 74, 435 772, 530330
Abstract:
The invention provides methods that employ derivatives of 2-cyano-6-hydroxy- or 2-cyano-6-amino-benzothiazole, for example, in a bioluminogenic reaction. Also provided are novel compounds that can be used in the methods. The invention further provides methods for detecting or determining the presence of molecules and/or enzymes, the modulator activity of such molecules, and/or the activity of such enzymes. The methods are adaptable to high-throughput format.

Bioluminescent Assays Using Cyanobenzothiazole Compounds

US Patent:
8592172, Nov 26, 2013
Filed:
Mar 11, 2011
Appl. No.:
13/046374
Inventors:
Jessica Kelts - Flint MI, US
Poncho Meisenheimer - San Luis Obispo CA, US
John Shultz - Verona WI, US
James J. Cali - Verona WI, US
Dongping Ma - Madison WI, US
Assignee:
Promega Corporation - Madison WI
International Classification:
C12Q 1/66
US Classification:
435 8, 435 72, 435 25, 435 74, 435 772
Abstract:
The invention provides methods that employ derivatives of 2-cyano-6-hydroxy- or 2-cyano-6-amino-benzothiazole, for example, in a bioluminogenic reaction. The invention further provides methods for detecting or determining the presence of molecules and/or enzymes, the modulator activity of such molecules, and/or the activity of such enzymes. The methods are adaptable to high-throughput format.

Luciferase-Based Assays

US Patent:
8603767, Dec 10, 2013
Filed:
Jan 25, 2013
Appl. No.:
13/750284
Inventors:
James J. Cali - Verona WI, US
Samuel Kin Sang Ho - New Bedford MA, US
Martha A. O'Brien - Madison WI, US
Richard Somberg - Madison WI, US
Robert F. Bulleit - Verona WI, US
Keith V. Wood - Mount Horeb WI, US
Assignee:
Promega Corporation - Madison WI
International Classification:
C12Q 1/66
C12Q 1/34
C12Q 1/37
US Classification:
435 8, 435 18, 435 23
Abstract:
A method and kit are provided for enhancing the tolerance of an assay reagent to compounds in an assay sample, the assay reagent including a luciferase enzyme. The method includes contacting the luciferase with a tolerance enhancement agent in an amount sufficient to substantially protect luciferase enzyme activity from interference of the compound and minimize interference by at least about 10% relative to an assay not having tolerance enhancement agent.

Isbn (Books And Publications)

Tqm For Purchasing Management

Author:
James F. Cali
ISBN #:
0070096236

Possible Relatives

David G Cali  Dean A Cali  Deborah C Cali  Dana A Cali  Debra A Cali  Daniel R Cali  Dennis D Cali  James V Johnson  James C Stamps  James E Stanage 

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