Work:
Genomics Institute of the Novartis Research Foundation - San Diego, CA 2011 to 2013
Postdoctoral fellow
Howard Hughes Medical Institute - New York, NY 2010 to 2011
Postdoctoral fellow
UC San Diego - San Diego, CA 2005 to 2010
Research Associate and Teaching Assistant, University of California at San Diego
Zhejiang University 2002 to 2005
Research Associate
Zhejiang University 2001 to 2002
Research Assistant
Education:
University of California at San Diego - San Diego, CA 2005 to 2010
PhD in Biology
Zhejiang University 2002 to 2005
Master in Cell Biology
Zhejiang University 1998 to 2002
Bachelor in Biology
Skills:
Cell Biology Techniques: High throughput screens and automation handling Mammalian tissue culture including human mesenchymal stem cells, hematopoietic stem cells, fibroblasts, subcutaneous preadipocytes, endothelial cells, and macrophages etc. Immunofluorescence and microscopy Cell separation using Miltenyi magnetic beads Cell-based assays including proliferation, cytotoxicity and apoptosis assays Flow cytometry Stable cell line establishment Cell-based luciferase assays In vitro target validation of small molecules and proteins Molecular Biology Techniques: ELISA SDS-PAGE and Western blot DNA/RNA extraction and RT-qPCR Microarray analysis Retrovirus production Protein expression and purification using bacteria and mammalian cells Lentivirus shRNA and siRNA knockdown Plasmid constructions In vivo Techniques: Histology and immunohistochemistry Xenograft tumor models and breast cancer models Collection and analysis of animal blood, serum and tissues Bone marrow reconstitution Intraperitoneal, subcutaneous and intravenous injections Development of animal models
