Inventors:
Teresa Bendele - Doylestown PA, US
Thomas Harbart - North Royalton OH, US
Dave Howard - North Ridgeville OH, US
Michael Kagan - Doylestown PA, US
Douglas Keene - North Wales PA, US
Dave Lapeus - Medina OH, US
Jared Mayes - Ambler PA, US
Douglas Paynter - Doylestown PA, US
Jerry Prohaska - Huntingdon Valley PA, US
Herman Rutner - Hatboro PA, US
International Classification:
C12Q 1/68
G06F 19/00
C12M 3/00
US Classification:
435006000, 435287200, 702020000, 702021000
Abstract:
The present invention provides a protocol and apparatus for enriching circulating tumor cells and other rare cells from blood, including debris and other components, from samples with high precision and at high throughput rates. This invention discloses an improved processing system from previously described semi-automated sample processing. The system further reduces operator intervention and hands-on time from prior systems. While this system has general utility in processing diverse materials, the system is configured for sample processing of biological specimens to provide an enriched fraction suitable for detection, enumeration and identification of target cells by appropriate analytical methodologies. The presence and quantities of such target cells in a sample specimen can utilized for screening and detection in disease such as cancer, assessing early stage pre-metastatic cancer, monitoring for disease remission in response to therapy and selection of more effective dose regimens or alternative therapies in case of relapse.