Jeffrey J Fredberg, 7879 Florence St UNIT 206S, Chestnut Hill, MA 02467

6440083, Aug 27, 2002

Jul 29, 1994

08/283074

Jeffrey J. Fredberg - Boston MA, 02215
Bruno Louis - 94010 Créteil, FR

A61B 5085

600533

Apparatus provides an output signal characteristic of the geometry of a confined volume. There is a conduit with an open first end for exchanging acoustical energy with the confined volume. An electroacoustical transducer is coupled to the conduit for launching acoustical energy into the conduit towards the opening in the confined volume producing an incident wave and a reflected wave to form a wave field representative of the geometry of the confined volume. In one embodiment, the acoustical energy is launched into a sidewall of the tube. At least first and second pressure-wave-sensing transducers are mounted along the length of the conduit in spaced relationship for providing first and second transduced signals representative of the wave field at spaced locations in the conduit. A processor processes the first and second transduced signals to provide an output signal characteristic of the geometry of the confined volume. In one embodiment, the processor provides high-pass filtering of the impulse response.

2011009, Apr 21, 2011

Aug 28, 2008

12/675882

Ramaswamy Krishnan - Cambridge MA, US
Chan Young Park - Cambridge MA, US
Jeffrey Fredberg - Sharon MA, US
Fei Liu - Warwick RI, US
Justin Mih - Mission Hill MA, US
Daniel Tschumperlin - Norfolk MA, US

C12Q 1/02
C12M 1/34

435 29, 4352887

Embodiments provide techniques for measuring and characterizing the dynamics of cell traction forces. Tunable elastic gel substrates can be disposed in multi-well plates. The gels can be of a uniform predetermined thickness. A multi-well plate can be loaded with gels of different shear moduli. An array of punch indenters can be attached to a loading platen such that the each indenter is aligned to a gel substrate. The indenters can apply tensile or compressive strains to the gel substrates. The magnitude, duration, and frequency of the strain can be controlled by a motor assembly coupled to a control system. The apparatus can be disposed in an incubator for long term cell culture experiments. The cell culture can be observed while a strain is applied. A ring-shaped indenter can be mounted on a microscope, coaxial to the objective lens, and lowered by a calibrated amount onto the underlying gel.

5532132, Jul 2, 1996

Sep 28, 1993

8/127977

Ning Wang - Wuhan, CN
Georgia Barlovatz-Meimon - Paris, FR
Jeffrey J. Fredberg - Sharon MA

President and Fellows of Harvard University - Cambridge MA
University of Paris

G01N 33567

435 721

The present invention is directed to a method for regulating formation of a complex of a plasminogen activator, its receptor and one of its inhibitors. More specifically, this method involves contacting a target cell having a plasminogen activator receptor with a compound which interacts with a component of the complex such that a change in target cell cytoskeletal stiffness results.

5666960, Sep 16, 1997

Mar 31, 1994

8/117176

Jeffrey Fredberg - Sharon MA
Gary Glass - Boston MA
John Lehr - Newton MA
Bruno Louis - Puteaux, FR

Hood Laboratories - Pembroke MA
Biomechanics Inc. - Boston MA

A61B 508

128716

Single hand supportable and operable apparatus for providing an output signal characteristic of the morphology of a respiratory tract includes an acoustic pipe for exchanging acoustical energy with the tract. The pipe has an open first end in communication with an opening in the respiratory tract. A transducer, such as a loudspeaker, is coupled to the pipe for launching acoustical energy into the pipe, producing an incident wave towards the opening in the tract and a reflected wave to form a transient wave field in the pipe representative of the morphology of the tract. Preferably, first and second pressure wave sensing transducers, such as microphones, mounted along the length of the pipe in spaced relationship provide first and second transduced signals representative of the transient wave field. A processor processes the first and second transduced signals to provide an output signal characteristic of the morphology of the tract, such as the cross-sectional area as a function of the distance from the opening in the tract.

4326416, Apr 27, 1982

Feb 11, 1980

6/120618

Jeffrey J. Fredberg - Sharon MA

Cambridge Collaborative, Inc. - Cambridge MA

G01N 2400

73597

A sound source located inside a tube having one end inside the mouth or nose of a subject provides an acoustic transient signal that propagates into the respiratory system of the subject to provide reflections sensed by a single mircophone inside the tube between the sound source and the mouth of the subject for recording on a transient recorder or computer and display on an oscilloscope after passing through a low pass filter to provide an output signal representative of the acoustical properties of the airways of the subject. This output signal may be processed in accordance with the deconvolution integral and other algorithms to determine essentially the acoustic impulse response, impedance or effective cross-sectional area of the airways, typically as a function of the distance from a reference point, such as the end of the tube.

5746699, May 5, 1998

Dec 18, 1996

8/769697

Jeffrey Fredberg - Sharon MA
Gary Glass - Boston MA
John Lehr - Newton MA
Bruno Louis - Puteaux, FR

Hood Laboratories - Pembroke MA
Biomechanics, Inc. - Boston MA

A61B 800

600529

Single hand supportable and operable apparatus for providing an output signal characteristic of the morphology of a respiratory tract includes an acoustic pipe for exchanging acoustical energy with the tract. The pipe has an open first end in communication with an opening in the respiratory tract. A transducer, such as a loudspeaker, is coupled to the pipe for launching acoustical energy into the pipe, producing an incident wave towards the opening in the tract and a reflected wave to form a transient wave field in the pipe representative of the morphology of the tract. Preferably, first and second pressure wave sensing transducers, such as microphones, mounted along the length of the pipe in spaced relationship provide first and second transduced signals representative of the transient wave field. A processor processes the first and second transduced signals to provide an output signal characteristic of the morphology of the tract, such as the cross-sectional area as a function of the distance from the opening in the tract.

5882314, Mar 16, 1999

Dec 17, 1991

7/808907

Jeffrey J. Fredberg - Sharon MA
Bruno Louis - Boston MA

Biomechanics, Inc. - Boston MA

A61B 508

600529

Apparatus provides an output signal characteristic of the geometry of a confined volume. There is a conduit with an open first end for exchanging acoustical energy with the confined volume. An electroacoustical transducer is coupled to the conduit for launching acoustical energy into the conduit towards the opening in the confined volume producing an incident wave and a reflected wave to form a transient wave field representative of the geometry of the confined volume. At least first and second pressure-wave-sensing transducers are mounted along the length of the conduit in spaced relationship for providing first and second transduced signals representative of the transient wave field at spaced locations in the conduit. A processor processes the first and second transduced signals to provide an output signal characteristic of the geometry of the confined volume.

5486457, Jan 23, 1996

Aug 25, 1993

8/112757

James P. Butler - Brookline MA
Jeffrey J. Fredberg - Sharon MA
Donald E. Ingber - Boston MA
Ning Wang - Brookline MA

Children's Medical Center Corporation - Boston MA
President and Fellows of Harvard College - Cambridge MA

G01N 3353

435 72

Mechanical stresses and deformations are applied directly to cell surface receptors or molecules and measured using a system including a magnetic twisting device in combination with ferromagnetic microbeads coated with ligands for integrins or any other surface receptors. The system can be used diagnostically to characterize cells and molecules and to determine the effect of transformation and compounds, including drugs, on the cells and molecules. The system can also be used to induce cells to grow or alter production of molecules by the cells.