Jesus N Ching, 56Watsonville, CA

6440725, Aug 27, 2002

Jun 25, 1999

09/331911

Farzad Pourahmadi - Fremont CA
William A. McMillan - Cupertino CA
Jesus Ching - Santa Clara CA
Ronald Chang - Redwood City CA
Lee A. Christel - Palo Alto CA
Gregory T. A. Kovacs - Stanford CA
M. Allen Northrup - Berkeley CA
Kurt E. Petersen - San Jose CA

Cepheid - Sunnyvale CA

C12M 134

4352885, 4352872, 4352886, 4353061, 4352865, 422 681, 422100, 422101, 422102

A cartridge ( ) for separating a desired analyte from a fluid sample includes a sample port ( ) and a sample flow path extending from the port through the body of the cartridge. The sample flow path includes at least one flow-through component ( ), e. g. , filter paper or a microfabricated chip, for capturing the desired analyte from the sample as the sample flows through the cartridge. The cartridge also includes an elution flow path for carrying elution fluid through the component ( ) to release captured analyte from the component into the elution fluid. The elution flow path diverges from the sample flow path after passing through the component ( ). Flow controllers ( A) and ( B) direct the remaining fluid sample into a waste chamber ( ) after the sample flows through the component ( ) and direct the elution fluid and eluted analyte into a reagent chamber ( ) and reaction chamber ( ). The continuous-flow design of the cartridge permits the rapid processing of a fluid sample that is larger in volume than any interactive region within the cartridge, allowing increased sensitivity in the detection of low copy concentrations of analytes, such as nucleic acid.

6664104, Dec 16, 2003

Nov 7, 2001

10/006850

Farzad Pourahmadi - Fremont CA
William A. McMillan - Cupertino CA
Jesus Ching - Santa Clara CA
Ronald Chang - Redwood City CA
Lee A. Christel - Palo Alto CA
Gregory T. A. Kovacs - Sanford CA
M. Allen Northrup - Berkeley CA
Kurt E. Petersen - Fremont CA

Cepheid - Sunnyvale CA

C12M 134

4352886, 4352872, 4352885, 422 70, 422101

A device for separating an analyte from a fluid sample comprises a cartridge incorporating a flow-through microfluidic chip. The microfluidic chip includes an extraction chamber having an array of microstructures for capturing the analyte and for subsequently releasing the captured analyte into an elution fluid. Each of the microstructures has an aspect ratio of at least 2:1. The cartridge also includes channels and at least one low controller (e. g. , one or more valves) for directing the flow of the sample and elution fluid through the microfluidic chip. The cartridge may optionally include a lysing region for lysing sample components (e. g. , cells spores, or microorganisms), a waste chamber for storing waste fluid, and reaction or detection chambers for amplifying or detecting the analyte.

6878540, Apr 12, 2005

Nov 7, 2001

10/006904

Farzad Pourahmadi - Fremont CA, US
William A. Mcmillan - Cupertino CA, US
Jesus Ching - Santa Clara CA, US
Ronald Chang - Redwood City CA, US
Christel A. Lee - Palo Alto CA, US
Gregory T.A. Kovacs - Stanford CA, US
Northrup M. Allen - Berkeley CA, US
Petersen E. Kurt - San Jose CA, US

Cepheid - Sunnyvale CA

C12M001/34

4352872, 4352885, 4352886, 4353061, 422 58, 422 681, 422101, 422104

A device for use with an ultrasonic transducer to lyse components of a fluid sample comprises a cartridge having a lysing chamber, an inlet port in fluid communication with the lysing chamber, and an outlet port for exit of the sample from the lysing chamber. The inlet and outlet ports are positioned to permit flow of the sample through the lysing chamber, and the chamber contains at least one solid phase for capturing the sample components to be lysed as the sample flows through the chamber. The lysing chamber is defined by at least one wall having an external surface for contacting the transducer to effect the transfer of ultrasonic energy to the chamber.

6881541, Apr 19, 2005

Mar 6, 2001

09/800590

Kurt E. Petersen - Santa Clara CA, US
Michael T. Taylor - Newark CA, US
Farzad Pourahmadi - Fremont CA, US
William A. McMillan - Cupertino CA, US
Ronald Chang - Redwood City CA, US
Stanley H. Sakai - Cupertino CA, US
Jesus Ching - Santa Clara CA, US
Douglas B. Dority - Mill Valley CA, US
Phillip Belgrader - Manteca CA, US
M. Allen Northrup - Berkeley CA, US

CEPHEID - Sunnyvale CA

C12Q001/68

435 6, 435 912, 435259, 436178, 536 254

A method for extracting nucleic acid from a fluid sample comprises the steps of introducing the sample into a cartridge having a sample flow path and a lysing chamber in the sample flow path. The lysing chamber contains at least one filter for separating cells or viruses from the sample. The sample is forced to flow through the lysing chamber to capture the cells or viruses with the filter, while used sample fluid flows to waste. The captured cells or viruses are disrupted to release their nucleic acid, the nucleic acid is eluted from the lysing chamber, and optionally the nucleic acid is amplified and detected in a reaction chamber of the cartridge.

6887693, May 3, 2005

Nov 7, 2001

10/006848

William A. McMillan - Cupertino CA, US
Kurt E. Petersen - San Jose CA, US
Lee A. Christel - Palo Alto CA, US
Ronald Chang - Redwood City CA, US
Farzad Pourahmadi - Fremont CA, US
Jesus Ching - Santa Clara CA, US
Gregory T. A. Kovacs - Stanford CA, US
M. Allen Northrup - Berkeley CA, US

Cepheid - Sunnyvale CA

C12N013/00

4351737, 4352867, 4352872, 4352885, 4352886

A device for lysing components (e. g. , cells, spores, or microorganisms) of a fluid sample comprises a cartridge having a lysing chamber for receiving the sample and having at least one solid phase in the lysing chamber for capturing the sample components to be lysed. An ultrasonic transducer is coupled to a wall of the lysing chamber to transfer ultrasonic energy to the captured sample components.

6893879, May 17, 2005

Nov 7, 2001

10/005685

Kurt E. Petersen - Fremont CA, US
William A. McMillan - Cupertino CA, US
Lee A. Christel - Palo Alto CA, US
Ronald Chang - Redwood City CA, US
Farzad Pourahmadi - Fremont CA, US
Jesus Ching - Santa Clara CA, US
Gregory T. A. Kovacs - Stanford CA, US
M. Allen Northrup - Berkeley CA, US

Cepheid - Sunnyvale CA

G01N001/18
C12Q001/68

436178, 435 6, 435 912, 4352872, 4352886, 435259

An analyte is separated from a fluid sample by introducing the sample into a cartridge having an extraction chamber containing capture material for capturing the analyte. The sample is forced to flow through the extraction chamber to capture the analyte with the capture material in the extraction chamber. The captured analyte is then eluted from the extraction chamber by forcing an elution fluid to flow through the extraction chamber. The cartridge may optionally include a lysing region for lysing sample components (e. g. , cells spores, or microorganisms), a waste chamber for storing waste fluid, and reaction or detection chambers for chemically reacting or detecting the eluted analyte.

7569346, Aug 4, 2009

May 3, 2005

11/121541

Kurt E. Petersen - San Jose CA, US
William A. McMillan - Cupertino CA, US
Lee A. Christel - Palo Alto CA, US
Ronald Chang - Redwood City CA, US
Farzad Pourahmadi - Fremont CA, US
Jesus Ching - Santa Clara CA, US
Gregory T. A. Kovacs - Stanford CA, US
M. Allen Northrup - Berkeley CA, US

Cepheid - Sunnyvale CA

C12Q 1/68

435 6, 435 912, 435259, 436178, 536 254

An analyte is separated from a fluid sample by introducing the sample into a cartridge having a sample port and a first flow path extending from the sample port. The first flow path includes an extraction chamber containing a solid support for capturing the analyte from the sample. The cartridge has a second flow path for eluting the captured analyte from the extraction chamber, the second flow diverging from the first flow path after passing through the extraction chamber. The sample is forced to flow through the extraction chamber and into a waste chamber, thereby capturing the analyte with the solid support as the sample flows through the extraction chamber. The captured analyte is then eluted from the extraction chamber by forcing an elution fluid to flow through the extraction chamber and along the second flow path.

7727473, Jun 1, 2010

Oct 17, 2006

11/582651

Jesus Ching - San Jose CA, US
David Hsiang Hu - Palo Alto CA, US
Steve Jia Chang Yu - San Jose CA, US
Phillip You Fai Lee - San Francisco CA, US

Progentech Limited

G01N 1/18
C12M 1/34

422 681, 422 63, 422100, 422101, 422102, 422104, 435 6, 4352872, 4352886, 4353061, 436 53

A cassette for preparing a sample is disclosed herein. The cassette includes a housing, which encloses the structures and the processes used to prepare the sample.