John W Atwood, 56Worcester, MA

7670848, Mar 2, 2010

Oct 18, 2005

11/251873

Michael R. Gambini - Bolton MA, US
John C. Voyta - Sudbury MA, US
John Atwood - Redding CT, US
Edward Lakatos - Bethel CT, US
Jeff Levi - Trumbull CT, US
Israel Metal - Flushing NY, US
George Sabak - Monroe CT, US
Yongdong Wang - Wilton CT, US

Applied Biosystems, LLC - Carlsbad CA

G01N 21/76
G01T 1/10

436172, 250361 C, 250361 R, 25055904, 25055905, 422 52, 422 8208, 436 50, 702 19, 702 25

In a luminescence detecting apparatus and method for analyzing luminescent samples, luminescent samples are placed in a plurality of sample wells in a tray, and the tray is placed in a visible-light impervious chamber containing a charge coupled device camera. In the chamber, light from the luminescent samples pass through a collimator, a Fresnel field lens, an infrared filter, and a camera lens, whereupon a focused image is created by the optics on the camera. The use of an infrared filter suppresses stray IR radiation resulting from plate phosphorescence (which can result in abnormally high backgrounds and/or alteration of the image received by the camera).

8278114, Oct 2, 2012

Mar 2, 2010

12/716219

Michael Gambini - Bolton MA, US
Jeff Levi - Trumbull CT, US
John Voyta - Sudbury MA, US
John Atwood - West Redding CT, US
Susan Atwood-Stone, legal representative - Charlotte VT, US
Bruce De Simas - Danville CA, US
Edward Lakatos - Croton On Hudson NY, US
Israel Metal - Flushing NY, US
George Sabak - Monroe CT, US
Yongdong Wang - Wilton CT, US

Applied Biosystems, LLC - Carlsbad CA

G01N 21/76
G01T 1/10

436172, 250361 C, 250361 R, 2503631, 250368, 356222, 356435, 422 52, 422 65, 422 67, 422 8208, 436 50, 702 19, 702 25

A luminescence detecting apparatus and method for analyzing luminescent samples is disclosed. A detecting apparatus may be configured so that light from luminescent samples pass through a collimator, a a first lens, a filter, and a camera lens, whereupon an image is created by the optics on the charge-coupled device (CCD) camera. The detecting apparatus may further include central processing control of all operations, multiple wavelength filter wheel, and/or a robot for handling of samples and reagents.

2003009, May 15, 2003

Dec 20, 2002

10/323669

Michael Gambini - Bolton MA, US
John Voyta - Sudbury MA, US
John Atwood - Redding CT, US
Bruce DeSimas - Danville CA, US
Edward Lakatos - Bethel CT, US
Jeff Levi - Trumbull CT, US
Israel Metal - Flushing NY, US
George Sabak - Monroe CT, US
Yongdong Wang - Wilton CT, US

G01N021/76

436/172000, 422/052000, 422/082080

A luminescence detecting apparatus and method for analyzing luminescent samples is disclosed. Luminescent samples are placed in a plurality of sample wells in a tray, and the tray is placed in a visible-light impervious chamber containing a charge coupled device camera. The samples may be injected in the wells, and the samples may be injected with buffers and reagents, by an injector. In the chamber, light from the luminescent samples pass through a collimator, a Fresnel field lens, a filter, and a camera lens, whereupon a focused image is created by the optics on the charge-coupled device (CCD) camera. The use of a Fresnel field lens, in combination with a collimator and filter, reduces crosstalk between samples below the level attainable by the prior art. Preferred embodiments of the luminescence detecting apparatus and method disclosed include central processing control of all operations, multiple wavelength filter wheel, and robot handling of samples and reagents. Preferred embodiments of processing software integrated with the invention include elements for mechanical alignment, outlier shaving, edge detection and masking, manipulation of multiple integration times to expand the dynamic range, crosstalk correction, dark subtraction interpolation and drift correction, multi-component analysis applications specifically tailored for luminescence, and uniformity correction.

2003014, Aug 7, 2003

Feb 20, 2003

10/370846

Michael Gambini - Bolton MA, US
John Atwood - Redding CT, US
Susan Stone - Charlotte VT, US
Judith Atwood - Redding CT, US
Eugene Young - Foster City CA, US
Edward Lakatos - Bethel CT, US
Anthony Cerrone - New Haven CT, US

C12M001/34

435/287200, 435/288400, 435/288700

An optical instrument monitors PCR replication of DNA in a reaction apparatus having a temperature cycled block with vials of reaction ingredients including dye that fluoresces in presence of double-stranded DNA. A beam splitter passes an excitation beam to the vials to fluoresce the dye. An emission beam from the dye is passed by the beam splitter to a CCD detector from which a processor computes DNA concentration. A reference strip with a plurality of reference emitters emit reference beams of different intensity, from which the processor selects an optimum emitter for compensating for drift. Exposure time is automatically adjusted for keeping within optimum dynamic ranges of the CCD and processor. A module of the beam splitter and associated optical filters is associated with selected dye, and is replaceable for different dyes.

2012030, Dec 6, 2012

Aug 13, 2012

13/584628

Michael Gambini - Bolton MA, US
John C. Voyta - Sudbury MA, US
John Atwood - West Redding CT, US
Edward Lakatos - Croton On Hudson NY, US
Jeff Levi - Trumbull CT, US
Israel Metal - Flushing NY, US
George Sabak - Monroe CT, US
Yongdong Wang - Wilton CT, US

LIFE TECHNOLOGIES CORPORATION - Carlsbad CA

G01N 21/76

436172, 422 52

A luminescence detecting apparatus and method for analyzing luminescent samples is disclosed. Luminescent samples are placed in a plurality of sample wells in a tray, and the tray is placed in a visible-light impervious chamber containing a charge coupled device camera. The samples may be injected in the wells, and the samples may be injected with buffers and reagents, by an injector. In the chamber, light from the luminescent samples pass through a collimator, a Fresnel field lens, a filter, and a camera lens, whereupon a focused image is created by the optics on the charge-coupled device (CCD) camera. The use of a Fresnel field lens, in combination with a collimator and filter, reduces crosstalk between samples below the level attainable by the prior art. Preferred embodiments of the luminescence detecting apparatus and method disclosed include central processing control of all operations, multiple wavelength filter wheel, and robot handling of samples and reagents. Preferred embodiments of processing software integrated with the invention include elements for mechanical alignment, outlier shaving, edge detection and masking, manipulation of multiple integration times to expand the dynamic range, crosstalk correction, dark subtraction interpolation and drift correction, multi-component analysis applications specifically tailored for luminescence, and uniformity correction.