John M Bowling Deceased1829 Pinnacle Rd, Pleasant View, TN 37146

5365455, Nov 15, 1994

Sep 20, 1991

7/763457

Clark Tibbetts - Nashville TN
John M. Bowling - Murfreesboro TN

Vanderbilt University - Nashville TN

G06G 758

364497

A method and system for automated nucleic acid sequence determination of a polynucleotide, wherein a nucleic acid sequencing ladder comprises signals corresponding to oligonucleotides formed from the polynucleotide, comprising the step of correlating, particularly in a trained neural network or a scatter plot, an intensity variable for each signal in the nucleic acid sequencing ladder with an informative variable for that signal, wherein the informative variable comprises information from at least two adjacent signals in the nucleic acid sequencing ladder, such that each signal in the nucleic acid sequencing ladder identified so as to determine the nucleic acid sequence corresponding to the polynucleotide. In particular, the relative separation between consecutive signals, the relative intensities between consecutive signals, and a pattern recognition factor, which incorporates a comparison of relative separations and intensities of at least two adjacent signals with pattern recognition templates, can be used as informative variables. Furthermore, this invention relates to a method and system for the on-the-fly resolution and extraction of information of signals contained in a digitized data stream involving calculation of the smoothed second derivative of a data point from the smoothed first derivative of the data point.