Julian C Borejdo, 765505 Willis Ave, Dallas, TX 75206

8159676, Apr 17, 2012

Jan 20, 2009

12/356288

Zygmunt Gryczynski - Fort Worth TX, US
Ignacy Gryczynski - Fort Worth TX, US
Evgenia Matveeva - Fort Worth TX, US
Julian Borejdo - Dallas TX, US

University of North Texas, Health Science Center at Fort Worth - Fort Worth TX

G01N 21/55

356445, 2504591, 250318, 250216, 2504581, 356 6, 356335, 356338, 356 36

The present invention includes methods for ratiometric detection of analytes by surface plasmon coupled emission detection that includes disposing a target on the metal layer of a surface plasmon resonance detection system; coupling a first analyte to a first fluorescent dye and a second analyte to a second fluorescent dye; contacting the first and second analytes to the target on the surface plasmon resonance detection system; and measuring the intensity of a first and a second surface plasmon resonance enhanced fluorescence emission ring, wherein the first and second rings, respectively, quantitatively represents the amount of first and second analyte within 50 nanometers of the metal surface.

2008023, Sep 25, 2008

Jan 22, 2008

12/018107

Zygmunt Gryczynski - Forth Worth TX, US
Ignacy Gryczynski - Fort Worth TX, US
Nils Calander - Goteborg, SE
Julian Borejdo - Dallas TX, US

University of North Texas Health Science Center at Fort Worth - Fort Worth TX

G01N 1/00

356 36

The present invention includes a microscope and a method for using the microscope for single molecule with reduced photobleaching of a fluorophore () that includes a light translucent material (); a metal layer () disposed on the light translucent material (); a medium () disposed on the metal layer (), the medium () having one or more fluorophores () capable of binding a target analyte (e.g., inside a cell); a microscope positioned to observe the surface plasmon emissions from the one or more fluorophores () within 50 nanometers of the surface of the metal layer (); an excitation source capable of exciting the one or more fluorophores (), the excitation source positioned to strike the light translucent material () at a first angle; and a light detector () that selectively detects emitted light generated by excited fluorophores () at a second angle (), wherein light emitted by the one or more fluorophores () at the surface plasmon angle is detected through the microscope, such that single molecules may be detected without significantly degrading fluorophore () emissions.

2011008, Apr 21, 2011

Oct 21, 2010

12/909628

Ignacy Gryczynski - Fort Worth TX, US
Zygmunt Gryczynski - Forth Worth TX, US
Rafal Luchowski - Fort Worth TX, US
Julian Borejdo - Dallas TX, US

University of North Texas Health Science Center at Forth Worth - Fort Worth TX

G01D 18/00
G01N 31/00
C09K 11/06

2502521, 2524081, 25230135

The present invention describes the development of thin film calibration strips for microscopy/spectroscopy systems and a simple method/routine to conduct instrument calibration using partially (uniaxially) oriented strip to calibrate microscopy system without the prior knowledge of exact polarization of the strip. The invention describes results from studies including a styryl derivative (LDS 798) embedded in poly(vinyl alcohol) (PVA) film. These films were progressively stretched up to 8 folds. Vertical and horizontal components of absorptions and fluorescence were measured and dichroic ratios were determined for different film stretching ratios. The stretched films have high polarization values for isotropic excitation. The isotropic and stretched PVA films doped with LDS 798 can be used as etalons in near infra red (NIR) spectroscopic measurements. The high polarization standards of the present invention have applications in NIR imaging microscopy where they can be used for correcting for instrumental factor in polarization measurements.

2011020, Aug 25, 2011

Feb 17, 2011

13/030048

Zygmunt Gryczynski - Fort Worth TX, US
Ignacy Gryczynski - Fort Worth TX, US
Rafal Luchowski - Fort Worth TX, US
Julian Borejdo - Dallas TX, US

University of North Texas Health Science Center at Fort Worth - Fort Worth TX

C40B 30/00
C12Q 1/02
C09K 11/06
C09K 11/02
H01F 1/00
H01L 31/00
B82Y 15/00

506 7, 435 29, 25230116, 25230135, 252 6251R, 2502141, 977773, 977832, 977834, 977902, 977927

The present invention provides partially fluorescent nanoparticles and methods of making and using the partially fluorescent nanoparticle having a nanoparticle with a matrix and a fluorescent dye dispersed in or about the matrix, wherein at least a portion of the fluorescent dye has been anisotropically bleached.

2015001, Jan 8, 2015

Jul 3, 2014

14/322998

- Fort Worth TX, US
Julian Borejdo - Dallas TX, US
Zygmunt Gryczynski - Fort Worth TX, US

G01N 21/64
G01N 33/58

506 9, 2504591, 2504581, 435 29, 435 5, 4352887, 506 39

The present invention includes a method and system for enhancing the signal-to-noise ratio in emission detection comprising: selecting a probe capable of at least one of fluorescence, phosphorescence, or delayed fluorescence in or about a sample that comprises interfering background signal; and exposing the probe to one or more controllable bursts, each burst comprising two or more pulses, wherein the one or more controllable bursts of high repetition energy pulses enhance the signal from the probe above that of the background signal.