Kristian B Keane, 47Salida, CO
Kristian Keane Phones & Addresses
Salida, CO
Chico, CA
Crested Butte, CO
Littleton, CO
Denver, CO
Hingham, MA
Boulder, CO
Winter Park, CO
Mentions for Kristian B Keane
Publications & IP owners
Us Patents
Methods And Compositions For Purification Of Nucleic Acid From A Host Cell
US Patent:
2004018, Sep 16, 2004
Filed:
Mar 12, 2003
Appl. No.:
10/387646
Inventors:
Michael Domanico - Longmont CO, US
Matt Meyers - Boulder CO, US
Kristian Keane - Boulder CO, US
Lisa Braun - Denver CO, US
Thomas Kolzau - Hamburg, DE
Matt Meyers - Boulder CO, US
Kristian Keane - Boulder CO, US
Lisa Braun - Denver CO, US
Thomas Kolzau - Hamburg, DE
International Classification:
G01N001/18
US Classification:
436/017000, 436/177000
Abstract:
Methods and compositions are provided for gently lysing and solubilizing cells. Methods and compositions are further provided for quickly purifying high quality low molecular weight nucleic acid from host cells. Target cells are treated with a pre-chilled lysis solution having a zwitterionic detergent, for example n-Dodecyl-N,N-dimethyl-3-ammonio-1-propanesulfonate, and a brief room-temperature incubation. Where nucleic acid purification is required, the lysis solution-treated cells are contacted with a nucleic acid capture matrix having an average pore size of at least about 1 m.
Methods And Compositions For Purification Of Nucleic Acid From A Host Cell
US Patent:
2009024, Oct 1, 2009
Filed:
Jan 22, 2009
Appl. No.:
12/358128
Inventors:
Michael J. Domanico - Longmont CO, US
Matt Meyers - Boulder CO, US
Kristian Keane - Boulder CO, US
Lisa R. Braun - Denver CO, US
Thomas Kolzau - Hamburg, DE
Matt Meyers - Boulder CO, US
Kristian Keane - Boulder CO, US
Lisa R. Braun - Denver CO, US
Thomas Kolzau - Hamburg, DE
Assignee:
Qiagen North American Holdings, Inc. - Valencia CA
International Classification:
C12N 1/08
US Classification:
435270
Abstract:
Methods and compositions are provided for gently lysing and solubilizing cells. Methods and compositions are further provided for quickly purifying high quality low molecular weight nucleic acid from host cells. Target cells are treated with a pre-chilled lysis solution having a zwitterionic detergent, for example n-Dodecyl-N,N-dimethyl-3-ammonio-1-propanesulfonate, and a brief room-temperature incubation. Where nucleic acid purification is required, the lysis solution-treated cells are contacted with a nucleic acid capture matrix having an average pore size of at least about 1 μm.
NOTICE: You may not use BackgroundCheck or the information it provides to make decisions about employment, credit, housing or any other purpose that would require Fair Credit Reporting Act (FCRA) compliance. BackgroundCheck is not a Consumer Reporting Agency (CRA) as defined by the FCRA and does not provide consumer reports.