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Kristopher M Zimmerman, 5048 Waterford Cir UNIT 108, Madison, WI 53719

Kristopher Zimmerman Phones & Addresses

3730 Karstens Dr, Madison, WI 53704   

Fitchburg, WI   

Andover, KS   

Pataskala, OH   

Marinette, WI   

Los Angeles, CA   

4362 De Volis Pkwy, Madison, WI 53711   

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Kristopher M Zimmerman

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Company: Promega Position: Research scientist iv

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Biotechnology

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Kristopher Zimmerman Photo 23

Research Scientist Iv

Location:
Madison, WI
Industry:
Biotechnology
Work:
Promega
Research Scientist Iv

Publications & IP owners

Wikipedia

Kristopher Zimmerman Photo 24

Kris Zimmerman

Kris Zimmerman is an American voice director, casting director, talent coordinator, and voice actor. Zimmerman is most known for directing the English ...

Us Patents

Synthetic Luciferases With Enhanced Light Output

US Patent:
8557970, Oct 15, 2013
Filed:
May 3, 2010
Appl. No.:
12/773002
Inventors:
Lance P. Encell - Fitchburg WI, US
Keith V. Wood - Mt. Horeb WI, US
Monika G. Wood - Mt. Horeb WI, US
Mary Hall - Waunakee WI, US
Paul Otto - Madison WI, US
Gediminas Vidugiris - Fitchburg WI, US
Kristopher Zimmerman - Madison WI, US
Assignee:
Promega Corporation - Madison WI
International Classification:
C12N 15/53
C12N 15/63
C12N 15/70
C12N 15/79
C12N 9/02
US Classification:
536 232, 435189, 4352523, 43525233, 4353201, 435810
Abstract:
A polynucleotide encoding a modified luciferase polypeptide. The modified luciferase polypeptide has at least 60% amino acid sequence identity to a wild-type luciferase and includes at least one amino acid substitution at a position corresponding to an amino acid in a wild-type luciferase of SEQ ID NO:1. The modified luciferase polypeptide has at least one of enhanced luminescence, enhanced signal stability, and enhanced protein stability relative to the wild-type luciferase.

Cell Lysis Composition, Methods Of Use, Apparatus And Kit

US Patent:
2004010, May 27, 2004
Filed:
Sep 26, 2003
Appl. No.:
10/673054
Inventors:
Laurie Engel - DeForest WI, US
John Shultz - Verona WI, US
Tonny Johnson - Madison WI, US
Kristopher Zimmerman - Madison WI, US
Laura Bozek - Madison WI, US
Judith Stevens - Oregon WI, US
Assignee:
Promega Corporation
International Classification:
C12N001/08
US Classification:
435/270000
Abstract:
Cell lysis compositions, methods for extracting and isolating proteins and peptides from a host cells using the compositions, kits and apparatus for extracting and isolating protein and peptide molecules from host cells and for detecting for the presence of a protein or peptide. The composition allows for the extraction and isolation of proteins and peptides from host cells without the need for mechanical disruption and with or without isolation of the cells from cell medium. The composition includes at least one surfactant having a hydrophobic-lipophilic balance value in the range from about 11 to about 16; and at least one cell membrane altering compound.

Cell Lysis Composition, Methods Of Use, Apparatus, And Kit

US Patent:
2007010, May 10, 2007
Filed:
Oct 31, 2006
Appl. No.:
11/590252
Inventors:
Laurie Engel - DeForest WI, US
John Shultz - Verona WI, US
Tonny Johnson - Madison WI, US
Kristopher Zimmerman - Madison WI, US
Laura Bozek - Madison WI, US
Judith Stevens - Oregon WI, US
Assignee:
Promega Corporation - Madison WI
International Classification:
C40B 40/10
C40B 30/06
C12P 21/06
US Classification:
435007100, 435069100, 435320100, 435325000, 435068100, 435348000, 435419000, 435252300, 435254200
Abstract:
Cell lysis compositions, methods for extracting and isolating proteins and peptides from a host cells using the compositions, kits and apparatus for extracting and isolating protein and peptide molecules from host cells and for detecting for the presence of a protein or peptide. The composition allows for the extraction and isolation of proteins and peptides from host cells without the need for mechanical disruption and with or without isolation of the cells from cell medium. The composition includes at least one surfactant having a hydrophobic-lipophilic balance value in the range from about 11 to about 16; and at least one cell membrane altering compound.

Mutant Protease Biosensors With Enhanced Detection Characteristics

US Patent:
2011028, Nov 17, 2011
Filed:
May 11, 2011
Appl. No.:
13/105648
Inventors:
Brock BINKOWSKI - Sauk City WI, US
Braeden BUTLER - Madison WI, US
Lance P. ENCELL - Fitchburg WI, US
Frank FAN - Verona WI, US
Brad HOOK - Baraboo WI, US
Paul OTTO - Madison WI, US
Gediminas VIDUGIRIS - Fitchburg WI, US
Susan WIGDAL - Belleville WI, US
Kristopher ZIMMERMAN - Madison WI, US
International Classification:
A01K 67/00
C07H 21/00
C12N 1/00
C12N 15/63
C12N 5/10
US Classification:
800 13, 4353201, 435325, 435243, 435419, 536 232
Abstract:
A polynucleotide encoding a biosensor polypeptide comprising a modified circularly-permuted thermostable luciferase and a linker linking the C-terminal portion of the thermostable luciferase to the N-terminal portion of the thermostable luciferase. The modified circularly-permuted thermostable luciferase is modified relative to a parental circularly-permuted thermostable luciferase. The linker contains a sensor region capable of interacting with a target molecule in a cell. The modified circularly-permuted thermostable luciferase has an enhanced response after interaction of the biosensor with the target molecule relative to the parental circularly-permuted thermostable luciferase in the presence of the target molecule. Alternatively, the modified circularly-permuted thermostable luciferase has an enhanced response after interaction of the biosensor with the target molecule relative to the modified circularly-permuted thermostable luciferase in the absence of the target molecule.

Mutant Protease Biosensors With Enhanced Detection Characteristics

US Patent:
2014003, Jan 30, 2014
Filed:
Nov 12, 2012
Appl. No.:
13/674655
Inventors:
Braeden BUTLER - Madison WI, US
Lance P. ENCELL - Fitchburg WI, US
Frank FAN - Verona WI, US
Brad HOOK - Baraboo WI, US
Paul OTTO - Madison WI, US
Kimberly RISMA - Cincinnati OH, US
Gediminas VIDUGIRIS - Fitchburg WI, US
Susan WIGDAL - Belleville WI, US
Kristopher ZIMMERMAN - Madison WI, US
Assignee:
PROMEGA CORPORATION - Madison WI
International Classification:
C12Q 1/66
C12N 9/02
US Classification:
435 613, 536 232, 4353201, 435 8, 435 618, 435325
Abstract:
A polynucleotide encoding a biosensor polypeptide comprising a modified circularly-permuted thermostable luciferase and a linker linking the C-terminal portion of the thermostable luciferase to the N-terminal portion of the thermostable luciferase. The modified circularly-permuted thermostable luciferase is modified relative to a parental circularly-permuted thermostable luciferase. The linker contains a sensor region capable of interacting with a target molecule in a cell. The modified circularly-permuted thermostable luciferase has an enhanced response after interaction of the biosensor with the target molecule relative to the parental circularly-permuted thermostable luciferase in the presence of the target molecule. Alternatively, the modified circularly-permuted thermostable luciferase has an enhanced response after interaction of the biosensor with the target molecule relative to the modified circularly-permuted thermostable luciferase in the absence of the target molecule.

Synthetic Oplophorus Luciferases With Enhanced Light Output

US Patent:
2022038, Dec 8, 2022
Filed:
Jun 20, 2022
Appl. No.:
17/844371
Inventors:
- Madison WI, US
Mary Hall - Waunakee WI, US
Paul Otto - Madison WI, US
Gediminas Vidugiris - Fitchburg WI, US
Keith V. Wood - Mt. Horeb WI, US
Monika G. Wood - Mt. Horeb WI, US
Kristopher Zimmerman - Madison WI, US
International Classification:
C12N 15/52
Abstract:
A polynucleotide encoding a modified luciferase polypeptide. The modified luciferase polypeptide has at least 60% amino acid sequence identity to a wild-type luciferase and includes at least one amino acid substitution at a position corresponding to an amino acid in a wild-type luciferase of SEQ ID NO: 1. The modified luciferase polypeptide has at least one of enhanced luminescence, enhanced signal stability, and enhanced protein stability relative to the wild-type luciferase.

Synthetic Oplophorus Luciferases With Enhanced Light Output

US Patent:
2021003, Feb 4, 2021
Filed:
Oct 20, 2020
Appl. No.:
17/075415
Inventors:
- Madison WI, US
Mary Hall - Waunakee WI, US
Paul Otto - Madison WI, US
Gediminas Vidugiris - Fitchburg WI, US
Keith V. Wood - Mt. Horeb WI, US
Monika G. Wood - Mt. Horeb WI, US
Kristopher Zimmerman - Madison WI, US
International Classification:
C12Q 1/66
C12N 9/02
Abstract:
A polynucleotide encoding a modified luciferase polypeptide. The modified luciferase polypeptide has at least 60% amino acid sequence identity to a wild-type luciferase and includes at least one amino acid substitution at a position corresponding to an amino acid in a wild-type luciferase of SEQ ID NO:1. The modified luciferase polypeptide has at least one of enhanced luminescence, enhanced signal stability, and enhanced protein stability relative to the wild-type luciferase.

Synthetic Oplophorus Luciferases With Enhanced Light Output

US Patent:
2020024, Aug 6, 2020
Filed:
Mar 17, 2020
Appl. No.:
16/821682
Inventors:
- Madison WI, US
Mary Hall - Waunakee WI, US
Paul Otto - Madison WI, US
Gediminas Vidugiris - Fitchburg WI, US
Keith V. Wood - Mt. Horeb WI, US
Monika G. Wood - Mt. Horeb WI, US
Kristopher Zimmerman - Madison WI, US
International Classification:
C12Q 1/66
C12N 9/02
Abstract:
A polynucleotide encoding a modified luciferase polypeptide. The modified luciferase polypeptide has at least 60% amino acid sequence identity to a wild-type luciferase and includes at least one amino acid substitution at a position corresponding to an amino acid in a wild-type luciferase of SEQ ID NO:1. The modified luciferase polypeptide has at least one of enhanced luminescence, enhanced signal stability, and enhanced protein stability relative to the wild-type luciferase.

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