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Bela F Asztalos, 7419 Louis W Farley Dr, Framingham, MA 01702

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19 Louis W Farley Dr, Framingham, MA 01702    508-8724619   

1242 Carrollton Ave, Metairie, LA 70005   

Middletown, OH   

New Orleans, LA   

Middletown, OH   

19 Louis W Farley Dr, Framingham, MA 01702   

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Us Patents

Methods For Separation And Immuno-Detection Of Biomolecules, And Apparatus Related Thereto

US Patent:
8470541, Jun 25, 2013
Filed:
Sep 26, 2009
Appl. No.:
12/567737
Inventors:
Bela F. Asztalos - Framingham MA, US
Ernst J. Schaefer - Natick MA, US
Assignee:
Boston Heart Diagnostics Corporation - Framingham MA
International Classification:
G01N 33/53
US Classification:
435 71
Abstract:
Disclosed are methods and apparatus for separation of biomolecules via two-dimensional gel electrophoresis, methods and apparatus for immunoblotting separated biomolecules, and methods for the use of biomolecules processed via the methods and apparatus of the present invention, including use in a clinical setting. The methods and apparatus for separation of biomolecules via two-dimensional gel comprises vertical agarose gel electrophoresis in the first dimension, and the electrophoresis of a novel non-denaturing 3-35% concave gradient polyacrylamide gel in the second dimension. This novel gel can be cast in a modified gel caster that can facilitate the pouring of multiple gels simultaneously. The methods and apparatus for immunblotting are useful with any type of immunoblotting, including Western blot, Northern blot, and Southern blot analyses. These methods and apparatus provide safe, efficient and cost-effective immunoblots, while facilitating the reduction of exposure to toxic or radioactive materials, as well as the disposal of those materials.

Methods For Separation And Immuno-Detection Of Biomolecules, And Apparatus Related Thereto

US Patent:
2017029, Oct 19, 2017
Filed:
Jun 30, 2017
Appl. No.:
15/639292
Inventors:
Bela F. Asztalos - Framingham MA, US
Ernst J. Schaefer - Natick MA, US
International Classification:
G01N 33/561
G01N 27/447
G01N 27/447
G01N 33/543
G01N 33/68
G01N 33/92
G01N 27/447
Abstract:
Disclosed are methods and apparatus for separation of biomolecules via two-dimensional gel electrophoresis, methods and apparatus for immunoblotting separated biomolecules, and methods for the use of biomolecules processed via the methods and apparatus of the present invention, including use in a clinical setting. The methods and apparatus for separation of biomolecules via two-dimensional gel comprises vertical agarose gel electrophoresis in the first dimension, and the electrophoresis of a novel non-denaturing 3-35% concave gradient polyacrylamide gel in the second dimension. This novel gel can be cast in a modified gel caster that can facilitate the pouring of multiple gels simultaneously. The methods and apparatus for immunoblotting are useful with any type of immunoblotting, including Western blot, Northern blot, and Southern blot analyses. These methods and apparatus provide safe, efficient and cost-effective immunoblots, while facilitating the reduction of exposure to toxic or radioactive materials, as well as the disposal of those materials.

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