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Lauren A Mcdaniel, 367911 111Th Ter E, Parrish, FL 34219

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Overland Park, KS   

Bradenton, FL   

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Us Patents

Method Of Identifying Severe Acute Respiratory Syndrome Corona Virus 2 (Sars-Cov-2) Ribonucleic Acid (Rna)

US Patent:
2021013, May 6, 2021
Filed:
Dec 30, 2020
Appl. No.:
17/137972
Inventors:
- Morrisville NC, US
Steven BRUZEK - St. Petersburg FL, US
Chelsea KNAPP - Clearwater FL, US
Lauren McDANIEL - St. Petersburg FL, US
Jessica SMALL - Oldsmar FL, US
Robert ULRICH - Oldsmar FL, US
Victoria WAGNER - Rochester Hills MI, US
International Classification:
C12Q 1/6865
C12Q 1/6853
Abstract:
A method for detecting the presence of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) in a biological sample includes the steps of lysing the biological sample to form a lysate and generating an amplified lysate by performing a nucleic acid sequence-based (NASBA) amplification for a target nucleic acid sequence in the lysate in the presence of: a forward primer having the oligonucleotide sequence selected from the group consisting of SEQ ID NO: 1, SEQ ID NO: 5, SEQ ID NO: 9, SEQ ID NO: 13, and SEQ ID NO: 17; a reverse primer having the oligonucleotide sequence selected from the group consisting of SEQ ID NO: 2, SEQ ID NO: 6, SEQ ID NO: 10, SEQ ID NO: 14, and SEQ ID NO: 18; and a molecular beacon having the oligonucleotide sequence selected from the group consisting of SEQ ID NO: 3, SEQ ID NO: 7, SEQ ID NO: 11, SEQ ID NO: 15, and SEQ ID NO: 19 and a fluorophore. The amplified lysate is exposed to an excitation source. A fluorescence of the fluorophore is detected in the amplified lysate exposed to the excitation source The SARS-CoV-2 is determined to be present in the biological sample in response to detecting the fluorescence of the fluorophore.

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