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Laurie A Rinehardt, 58Denver, CO

Laurie Rinehardt Phones & Addresses

Evergreen, CO   

1301 Red Tail Dr, Verona, WI 53593    608-8457303   

2427 Eagleview Cir, Longmont, CO 80504    303-7020161   

Kenosha, WI   

Wilmington, DE   

Superior, CO   

Evergreen, CO   

Superior, CO   

Jefferson, CO   

Mentions for Laurie A Rinehardt

Publications & IP owners

Us Patents

Detecting And Amplifying Target Nucleic Acids Using Exonucleolytic Activity

US Patent:
5573907, Nov 12, 1996
Filed:
Aug 4, 1993
Appl. No.:
8/101877
Inventors:
John J. Carrino - Gurnee IL
Uwe Spies - Limburg, DE
Laurie A. Rinehardt - Kenosha WI
Edward K. Pabich - Chicago IL
Assignee:
Abbott Laboratories - Abbott Park IL
International Classification:
C12Q 168
C12P 1934
C07H 2104
US Classification:
435 6
Abstract:
The present invention relates to improved LCR amplification schemes using at least one downstream probe modified at its 5' end to reduce or eliminate target independent amplification. The different modified probes, and kits containing them are also presented. Also presented is a method for detecting differences in nucleic acid sequences, with reduced target independent amplification, using the modified probes.

Amplification Of Target Nucleic Acids Using Gap Filling Ligase Chain Reaction

US Patent:
5427930, Jun 27, 1995
Filed:
Jun 28, 1991
Appl. No.:
7/722798
Inventors:
Larry G. Birkenmeyer - Chicago IL
John J. Carrino - Gurnee IL
Bruce J. Dille - Antioch IL
Hsiang-Yun Hu - Libertyville IL
Jon D. Kratochvil - Kenosha WI
Thomas G. Laffler - Libertyville IL
Ronald L. Marshall - Zion IL
Laurie A. Rinehardt - Kenosha WI
Natalie A. Solomon - Buffalo Grove IL
Assignee:
Abbott Laboratories - Abbott Park IL
International Classification:
C12P 1934
C12Q 168
C07H 2104
US Classification:
435 9152
Abstract:
An improved, "gap filling" embodiment of the Ligase Chain Reaction (LCR) is described. Gap filling LCR is LCR wherein at least one of the probes is recessed so that a gap is formed between the adjacent probes when they are hybridized to target. The gap is filled using polymerase and deoxyribonucleotide triphosphates before ligation of the probes together. There are single and double gap versions, depending on whether one or two probes are recessed and require filling before ligation. The improvement resides in selecting and using target sequences such that only a single type, or two types, of deoxyribonucleotide triphosphate(s) are required to fill double gaps each being 1-10 bases in length, preferably 1-3 bases. Probes having specific sequences are claimed for a number of pathogens.

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