Leroy A Wheeler DeceasedEllicott, MD

Company: Washington university in st. louis May 2008 Position: Postdoctoral research scholar

INTERPERSONAL AND COMMUNICATION SKILL SET: a positive attitude • an empathetic nature • and the ability to build rapport and trust among my colleagues. Speaking clearly • succinctly and in an open and non-confrontational manner. Possessing a team player mentality • where success of the team is the primary goal and is accomplished when team members work together • have a willingness to share knowledge • take the initiative to tackle difficult problems and understand team dynamics. IMMUNOLOGY SKILL SET: 10-color flow cytometry analysis • lymphocyte • goblet cell and dendritic cell isolation from mouse intestine • FACS sorting and in vitro cell culture of sorted cells • ELISA • ELISPOT • qRT-PCR • 4-color fluorescence microscopy • histology • mouse husbandry and genotyping • software including FlowJo • GraphPad Prism and Adobe Illustrator. CELL AND MOLECULAR BIOLOGY SKILL SET: Tissue culture • bacteria cell culture • cytotoxicity and cell viability via MTT assay • endotoxin testing via LAL assay • subcloning • site directed mutagenesis • plasmid propagation • cell transfection • immunoblot • PCR • immunocytochemistry • in vitro kinase activity • immunoprecipitation • mammalian cell fractionation • 3H-Thymidine incorporation.