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Fang Zhang, 5910 Providence Dr, Flemington, NJ 08822

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10 Providence Dr, Flemington, NJ 08822   

1 Bradford Ave, Trenton, NJ 08610   

Los Alamos, NM   

Gainesville, FL   

Mentions for Fang Zhang

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Fang Zhang Photo 1

Fang Zhang - Lawyer

Specialties:
Contracts & Agreements, Corporate & Incorporation, Entertainment, Entertainment, Estate Planning, Intellectual Property, Real Estate
ISLN:
922383671
Admitted:
2011

Publications & IP owners

Us Patents

Method Of Sequence Optimization For Improved Recombinant Protein Expression Using A Particle Swarm Optimization Algorithm

US Patent:
8326547, Dec 4, 2012
Filed:
Sep 30, 2010
Appl. No.:
12/894401
Inventors:
Xiaowu Liu - Nanjing, CN
Yun He - Nanjing, CN
Zhuying Wang - Monmouth Junction NJ, US
Chunjiao Wang - Nanjing, CN
Zhibing Liu - Nanjing, CN
Tianhui Xia - Edison NJ, US
Luquan Wang - East Brunswick NJ, US
Fang Liang Zhang - Fanwood NJ, US
Assignee:
Nanjingjinsirui Science & Technology Biology Corp. - Nanjing
International Classification:
G06F 19/10
G06F 19/00
US Classification:
702 19, 702 20
Abstract:
An improved gene sequence optimization method, the systematic optimization method, is described for boosting the recombinant expression of genes in bacteria, yeast, insect and mammalian cells. This general method takes into account of multiple, preferably most or all, of the parameters and factors affecting protein expression including codon usage, tRNA usage, GC-content, ribosome binding sequences, promoter, 5′-UTR, ORF and 3′-UTR sequences of the genes to improve and optimize the gene sequences to boost the protein expression of the genes in bacteria, yeast, insect and mammalian cells. In particular, the invention relates to a system and a method for sequence optimization for improved recombinant protein expression using a particle swarm optimization algorithm. The improved systematic optimization method can be incorporated into a software for more efficient optimization.

Homologous Recombination-Based Dna Cloning Compositions

US Patent:
8501454, Aug 6, 2013
Filed:
Jan 31, 2012
Appl. No.:
13/362382
Inventors:
Weiqiang Liu - Nanjing, CN
Ping Yang - Princeton NJ, US
Tao Wang - Nanjing, CN
Zhuying Wang - Monmouth Junction NJ, US
Wenzhu Chen - Nanjing, CN
Fang Liang Zhang - Fanwood NJ, US
Assignee:
Nanjingjinsirui Science & Technology Biology Corp. - Nanjing
International Classification:
C12N 1/20
C12N 9/18
C07K 17/00
C12N 15/64
US Classification:
4352521, 435197, 435 914, 530350
Abstract:
Methods and compositions for cloning a donor DNA molecule into an acceptor vector at a predetermined location are described. The methods are based on homologous recombination mediated by in vitro treatment of the donor DNA and the acceptor vector with an enzyme cocktail containing an exonuclease and a single-stranded DNA binding protein.

Homologous Recombination-Based Dna Cloning Methods And Compositions

US Patent:
2010006, Mar 11, 2010
Filed:
Sep 10, 2009
Appl. No.:
12/584684
Inventors:
Weiqiang Liu - Nanjing, CN
Ping Yang - Princeton NJ, US
Tao Wang - Nanjing, CN
Ping Yang - Nanjing, CN
Zhuying Wang - Monmouth Junction NJ, US
Wenzhu Chen - Nanjing, CN
Fang Liang Zhang - Fanwood NJ, US
Assignee:
GenScript Corporation - Piscataway NJ
International Classification:
C12N 15/64
C12N 9/16
US Classification:
435 914, 435196
Abstract:
Methods and compositions for cloning a donor DNA molecule into an acceptor vector at a predetermined location are described. The methods are based on homologous recombination mediated by in vitro treatment of the donor DNA and the acceptor vector with an enzyme cocktail containing an exonuclease and a single-stranded DNA binding protein.

Homologous Recombination-Based Dna Cloning Methods And Compositions

US Patent:
2010016, Jul 1, 2010
Filed:
Mar 5, 2010
Appl. No.:
12/660885
Inventors:
Weiqiang Liu - Nanjing, CN
Ping Yang - Princeton NJ, US
Tao Wang - Nanjing, CN
Zhuying Wang - Monmouth Junction NJ, US
Wenzhu Chen - Nanjing, CN
Fang Liang Zhang - Fanwood NJ, US
Assignee:
GENSCRIPT HOLDINGS (Hong Kong) LIMITED - Piscataway NJ
International Classification:
C12P 19/34
C12N 15/66
US Classification:
435 912, 435 9141
Abstract:
Methods and compositions for cloning a donor DNA molecule into an acceptor vector at a predetermined location are described. The methods are based on homologous recombination mediated by in vitro treatment of the donor DNA and the acceptor vector with an enzyme cocktail containing an exonuclease and a single-stranded DNA binding protein.

Rapid Electrophoresis Binding Method And Related Kits And Compositions

US Patent:
2010032, Dec 30, 2010
Filed:
Jun 9, 2010
Appl. No.:
12/797011
Inventors:
Zhuying WANG - Monmouth Junction NJ, US
Jinyu YANG - Nanjing, CN
Fanli MENG - New York NY, US
Bing LAI - Nanjing, CN
Zhan DAI - Nanjing, CN
Fang Liang ZHANG - Fanwood NJ, US
Assignee:
GENSCRIPT HOLDINGS (HONG KONG) LIMITED - Piscataway NJ
International Classification:
G01N 27/26
US Classification:
204461, 204456
Abstract:
An improved staining method is described for staining a biopolymer such as a peptide, a protein, an RNA, a DNA, an oligosaccharide or a complex containing a peptide, a protein, an RNA, a DNA, or an oligosaccharide in a matrix. The method includes the step of moving a staining reagent into the matrix using an electric force. The staining time can be dramatically reduced relative to conventional technologies. The improved staining method can particularly be used, for example, to stain proteins after gel separation. Other related methods and related kits are also described.

Enzymatic Method For Preparing Aspartam

US Patent:
2012029, Nov 22, 2012
Filed:
May 17, 2012
Appl. No.:
13/473993
Inventors:
Zhuying WANG - Monmouth Junction NJ, US
Jian SUN - Nanjing, CN
Xiangqun LI - Lutherville-Timonium MD, US
Fang Liang ZHANG - Fanwood NJ, US
Assignee:
GENSCRIPT NANJING - Nanjing
International Classification:
C12P 21/02
G01N 27/62
US Classification:
435 23, 435 681
Abstract:
An improved method is described for the synthesis of aspartame using a condensation reaction between the alpha-carboxyl group of the L-aspartic acid and the amino group of the methyl L-phenylalaninate catalyzed by an enzyme. The method allowed efficient and cost effective production of aspartame. A method of identifying an enzyme useful for preparing aspartame is also described.

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