Inventors:
George Michael Alsop - South Charleston WV
Assignee:
Union Carbide Corporation - New York NY
International Classification:
C12K 100
Abstract:
Method of and apparatus for testing materials for levels of agents that are toxic and/or inhibitory to microorganisms involving (a) the mixing of a sample of said material with a liquid medium containing microorganisms, said medium being capable of supporting growth of said microorganisms; (b) the impregnating of the resulting liquid mixture into a porous inert support mass capable of permitting oxygen transfer from ambient gas enveloping said mass to said mixture impregnated into it; (c) the incubating of said mixture impregnated into said mass for a period of time and under conditions sufficient to promote bacterial growth therein in the absence of toxic or inhibitory agents; (d) the removing of the incubated mixture from said inert support mass and filtering same through an open pore filter; and (e) the observing of the degree of turbidity, or lack of it, in the resulting filtrate. In particular, the apparatus of the invention comprises (1) a plurality of closed test reactors; (2) a porous inert support mass disposed within each said reactor and capable of being impregnated with a liquid test mixture and permitting transfer of oxygen from ambient oxygen-containing gas enveloping said mass to said mixture; and (3) a closed source of oxygen connected to said closed test reactor to provide oxygen thereto.