Ming C Gu, 66416 Schindler Dr, Yardley, PA 19067

6770876, Aug 3, 2004

Apr 11, 2003

10/412191

Ming Gu - Yardley PA
Wenjeng Li - Plainsboro NJ
John W. Allen - Eastampton NJ

Bristol-Myers Squibb Company - Princeton NJ

B01D 1508

250288, 73 6159, 2101982

The present invention relates to an autosampler device useful in high pressure liquid chromatography (HPLC), and more particularly to a device useful for the automated introduction of small sample volumes into a HPLC system. Methods of analyzing low abundant protein samples using such a device are also included.

7348553, Mar 25, 2008

Oct 28, 2005

11/261440

Yongdong Wang - Wilton CT, US
Ming Gu - Yardley PA, US

Cerno Bioscience LLC - Danbury CT

H01J 49/26
B01D 59/44
B01D 15/08

250282, 250281, 250286, 250424, 702 27, 702 28, 702 85

A method for calibrating and analyzing data from a mass spectrometer, comprising the steps of acquiring raw profile mode data containing mass spectral responses of ions with or without isotopes; calculating theoretical isotope distributions for each of at least one calibration ion based on elemental composition; convoluting the theoretical isotope distributions with an initial peak shape function to obtain theoretical isotope profiles for each ion; constructing a peak component matrix including the theoretical isotope profiles for calibration ions as peak components; performing a regression analysis between the raw profile mode mass spectral data and the peak component matrix; and reporting the regression coefficients as the relative concentrations for each of the components. A mass spectrometry system operated in accordance with the method and a computer readable medium having program code thereon for performing the method.

7451052, Nov 11, 2008

May 30, 2006

11/444158

Yongdong Wang - Wilton CT, US
Ming Gu - Yardley PA, US

Cerno Bioscience LLC - Danbury CT

H01J 49/00

702 85, 702 76

A method of performing mass spectral analysis involving at least one of the isotope satellites of at least one ion, comprising acquiring a measured mass spectral response including at least one of the isotope satellites; constructing a peak component matrix with mass spectral response functions; performing a regression analysis between the acquired mass spectral response and the peak component matrix; and reporting one of statistical measure and regression coefficients from the regression analysis for at least one of mass spectral peak purity assessment, ion charge determination, mass spectral deconvolution, and mass shift compensation. A method for the identification of an ion in a sample through acquired MS scans, comprising obtaining an isotope pattern of an ion; constructing a projection matrix based on the isotope pattern or MS scan; projecting the isotope pattern or MS scan onto the projection matrix to calculate at least one of projection residual and projected data; and performing a statistical test on at least one of the projection residual and projected data to determine if the ion exists in the sample or if there is interference. A method which takes advantage of mass defect or isotope pattern analysis, and software and hardware for implementing all aspects of the invention.

7460649, Dec 2, 2008

Jan 14, 2005

11/035422

Feng Ouyang - Holmdel NJ, US
Patrick Duvaut - Tinton Falls NJ, US
Ming Gu - Eatontown NJ, US

Conexant Systems, Inc. - Newport Beach CA

H04M 1/24
H04M 3/08
H04M 3/22

379 104, 379 103, 379 2901

A method and apparatus for single end loop testing for DSL provisioning and maintenance provides testing of the DSL loop using digital techniques including frequency compensation, matched filtering and median filtering, to remove strong background and enhance desired signal for peak detection. The SELT system allows DSL providers to extract loop information from reflective measurements at the central office (CO) so as to determine loop capability of supporting different DSL services. The method and apparatus acquires the desired signal (the far-end echo) which is submerged in the correlated background noise (the near-end echo) by providing a process for detecting loop length and termination types with high accuracy for loops up to 12 kft for 24 AWG and up to 9 kft for 26 AWG by exploiting several digital signal processing techniques including frequency.

7493225, Feb 17, 2009

Oct 20, 2004

10/576564

Yongdong Wang - Wilton CT, US
Ming Gu - Yardley PA, US

Cerno Bioscience LLC - Danbury CT

G01R 35/00

702 85, 250282, 250287, 436 58, 702 23, 702 27, 702104, 702109, 702189

A method for obtaining at least one calibration filter for a Mass Spectrometry (MS) instrument system. Measured isotope peak cluster data in a mass spectral range is obtained for a given calibration standard. Relative isotope abundances and actual mass locations of isotopes corresponding thereto are calculated for the given calibration standard. Mass spectral target peak shape functions centered within respective mass spectral ranges are specified. Convolution operations are performed between the calculated relative isotope abundances and the mass spectral target peak shape functions to form calculated isotope peak cluster data. A deconvolution operation is performed between the measured isotope peak cluster data and the calculated isotope peak cluster data after the convolution operations to obtain the at least one calibration filter. Provisions are made for normalizing peak widths, combining internal and external calibration, and using selected measured peaks as standards. Aspects of the methods are applied to other analytical instruments.

7781729, Aug 24, 2010

May 27, 2007

11/754305

Yongdong Wang - Wilton CT, US
Ming Gu - Yardley PA, US

Cerno Bioscience LLC - Danbury CT

B01D 59/44
H01J 49/00

250282, 250281, 250283, 250287, 250288, 250289, 250290, 250291, 250292, 702 22, 702 23, 702 31, 702 76, 702 85

A method for analyzing data from a mass spectrometer comprising obtaining calibrated continuum spectral data by processing raw spectral data; obtaining library spectral data which has been processed to form calibrated library data; and performing a least squares fit, preferably using matrix operations (equation 1), between the calibrated continuum spectral data and the calibrated library data to determine concentrations of components in a sample which generated the raw spectral data. A mass spectrometer system (FIG. ) that operates in accordance with the method, a data library of transformed mass spectra, and a method for producing the data library.

7904253, Mar 8, 2011

Jul 30, 2007

11/830772

Yongdong Wang - Wilton CT, US
Ming Gu - Yardley PA, US

Cerno Bioscience LLC - Danbury CT

G06F 19/00

702 27, 702 28, 702 85, 250281, 250282, 250286, 250424

A method for determining elemental composition of ions from mass spectral data, comprising obtaining at least one mass measurement from mass spectral data; obtaining a search list of candidate elemental compositions whose exact masses fall within a given mass tolerance range from the accurate mass; reporting a probability measure based on a mass error; calculating an isotope pattern for each candidate elemental composition from the search list; constructing a peak component matrix including at least one of the isotope pattern and mass spectral data; performing a regression against at least one of isotope pattern, mass spectral data, and the peak component matrix; reporting a second probability measure for at least one candidate elemental composition based on the isotope pattern regression; and combining the two the probability measures into an overall probability measure. A method for determining elemental isotope ratios from mass spectral data.

8010306, Aug 30, 2011

Feb 16, 2009

12/371759

Yongdong Wang - Wilton CT, US
Ming Gu - Yardley PA, US

Cerno Bioscience LLC - Danbury CT

G01R 35/00

702 85, 250282, 250287, 436 58, 702 23, 702104, 702109, 702189

A method for obtaining at least one calibration filter for a Mass Spectrometry (MS) instrument system. Measured isotope peak cluster data in a mass spectral range is obtained for a given calibration standard. Relative isotope abundances and actual mass locations of isotopes corresponding thereto are calculated for the given calibration standard. Mass spectral target peak shape functions centered within respective mass spectral ranges are specified. Convolution operations are performed between the calculated relative isotope abundances and the mass spectral target peak shape functions to form calculated isotope peak cluster data. A deconvolution operation is performed between the measured isotope peak cluster data and the calculated isotope peak cluster data after the convolution operations to obtain the at least one calibration filter. Provisions are made for normalizing peak widths, combining internal and external calibration, and using selected measured peaks as standards. Aspects of the methods are applied to other analytical instruments.