Paul C Grossman, 37115 Chevy Chase, Thermopolis, WY 82443

6627433, Sep 30, 2003

Aug 24, 2001

09/938767

Jeffery D. Frazier - Redwood City CA
Paul D. Grossman - Hillsborough CA

Applera Corporation - Foster City CA

C12M 134

4352887, 4352871, 4352882, 4352884, 436165, 204450

A side-entry excitation arrangement is provided with a multi-channel analyte-separation device. In various embodiments, a plurality of channels are disposed in an array, with a laser disposed to direct an excitation beam of light along a beam path that crosses the longitudinal axes of the channels, so as to simultaneously irradiate a region of each of the channels. Devices of the invention can be useful, for example, in the separation and analysis of bio-molecules, such as DNA, RNA, etc.

5240576, Aug 31, 1993

Jan 5, 1990

7/461568

Henk H. Lauer - Belmont CA
Paul D. Grossman - Redwood City CA
Dennis E. Mead - Campbell CA

Applied Biosystems, Inc. - Foster City CA

G01N 2726
B01D 5702

2041801

an apparatus is disclosed for providing capillary electrophoresis, which includes an electronically controlled valve system for automatically introducing a sample into the capillary by means of a vacuum at the end of the capillary tube. This approach of sucking in the sample is extremely accurate and reproducible, and results in a minimum of band broadening. Furthermore, it enables the entire capillary electrophoresis sytem to be easily automated. An automated temperature control system is provided which enables the temperature of the capillary tube (and hence the solvent/solute system) to be controlled during electrophoresis, thereby very directly controlling pH and electrophoretic mobility. In another embodiment, the capillary is prewashed and equilibrated to achieve substantially zero charge on the capillary wall, thereby essentially eliminating electroosmotic flow and substantially improving resolution.

5346999, Sep 13, 1994

Mar 24, 1989

7/328471

Guy R. Cathcart - Berkeley CA
Paul D. Grossman - Foster City CA
P. Eric Mayrand - Pacifica CA
Eric S. Nordman - San Bruno CA
Norman M. Whiteley - San Carlos CA

Applied Biosystems, Inc. - Foster City CA

C07H 2300

536 2541

An automated apparatus is provided which implements a new method of extracting and purifying nucleic acids from cells without the use of centrifugation. In the method, a lysate is created by treating the cells with proteinase K in the presence of a lysis buffer having a high concentration of a salt. The lysate is mixed with a phenol-based solvent system, thereby creating an emulsion. The emulsion is heated to promote phase separation. Similarly, the rate of phase separation is also enhanced by increasing the surface area of the emulsion. Once the phase separation is complete, the lower organic phase is removed and the upper aqueous phase is repeatedly extracted with the phenol-based solvent a preselected number of times, and is finally extracted using chloroform. The remaining aqueous phase is then dialyzed to further purify and concentrate the nucleic acid solution. Two preferred embodiments of apparatus are presented to accomplish this extraction.

5207886, May 4, 1993

Sep 27, 1991

7/767345

Henk H. Lauer - Belmont CA
Paul D. Grossman - Redwood City CA
Dennis E. Mead - Campbell CA

Applied Biosystems, Inc. - Foster City CA

G01N 2726
G01N 27447
B01D 5702

204299R

An apparatus is disclosed for providing capillary electrophoresis, which includes an electronically controlled valve system for automatically introducing a sample into the capillary by means of a vacuum at the end of the capillary tube. This approach of sucking in the sample is extremely accurate and reproducible, and results in a minimum of band broadening. Furthermore, it enables the entire capillary electrophoresis system to be easily automated. An automated temperature control system is provided which enables the temperature of the capillary tube (and hence the solvent/solute system) to be controlled during electrophoresis, thereby very directly controlling pH and electrophoretic mobility. In another embodiment, the capillary is prewashed and equilibrated to achieve substantially zero charge on the capillary wall, thereby essentially eliminating electroosmotic flow and substantially improving resolution.

5597468, Jan 28, 1997

Oct 17, 1994

8/324359

Henk H. Lauer - Belmont CA
Paul D. Grossman - Redwood City CA
Dennis E. Mead - Campbell CA

The Perkin-Elmer Corporation - Foster City CA

G01N 2726
G01N 27447

204604

An apparatus is disclosed for providing capillary electrophoresis, which includes an electronically controlled valve system for automatically introducing a sample into the capillary by means of a vacuum at the end of the capillary tube. This approach of sucking in the sample is extremely accurate and reproducible, and results in a minimum of band broadening. Furthermore, it enables the entire capillary electrophoresis sytem to be easily automated. An automated temperature control system is provided which enables the temperature of the capillary tube (and hence the solvent/solute system) to be controlled during electrophoresis, thereby very directly controlling pH and electrophoretic mobility. In another embodiment, the capillary is prewashed and equilibrated to achieve substantially zero charge on the capillary wall, thereby essentially eliminating electroosmotic flow and substantially improving resolution.