Ralph A Eatz, 811350 Treebrook Ct, Roswell, GA 30075

5468618, Nov 21, 1995

Aug 18, 1993

8/109011

Lyle T. Sinor - Roswell GA
Ralph A. Eatz - Roswell GA
Darryl L. Stone - Roswell GA
Fred V. Plapp - Overland Park KS

Immucor, Inc. - Norcross GA

G01N 33543
G01N 33554
G01N 33555

435 721

An article for performing solid-phase immunological assays and methods for performing assays utilizing such article. A solid-phase support suitable for immunological assays is stained with an organic dye that has a net-positive charge and a hydrophobic aromatic ring structure. An immunologically reactive component having a net-negative charge, such as a whole cell, is securely immobolized by the dye to the support through noncovalent interactions. A sample of biological fluid may be added to the article to which the immunologically reactive component has been immobilized to effect binding of antigens or antibodies in the sample which are specific to the immobilized component. The presence of the antigen-antibody complex may then be determined by any known means.

5192663, Mar 9, 1993

May 21, 1991

7/702888

Lyle T. Sinor - Roswell GA
Ralph A. Eatz - Roswell GA

Immucor, Inc. - Norcross GA

G01N 33543

435 725

A method for drying mammalian cells, such as erythrocytes, lymphocytes, leukocytes and platelets onto a solid-phase support for use in solid-phase immunoassays through use of a drying solution and an article for use in immunoassays prepared by such method. The method comprises immobilizing a monolayer of cells onto the solid-phase support by non-covalent binding. This is accomplished by staining the solid-phase support with an organic dye having a net positive charge which permits non-covalent binding of the cells which carry a net negative charge to the solid-phase support. These cells are dried or fixed to the solid-phase support by addition of a drying solution which comprises an aqueous solution of a monosaccharide, disaccharide, trisaccharide or cyclitol and a salt. The preferred monosaccharide is D-(-)glucose and the preferred salt is sodium chloride. The preferred drying solution comprises a 1.

5030560, Jul 9, 1991

Nov 4, 1988

7/267014

Lyle T. Sinor - Roswell GA
Ralph A. Eatz - Roswell GA

Immucor, Inc. - Norcross GA

G01N 33543
G01N 33544
G01N 33552

435 721

A method for drying mammalian cells, such as erythrocytes, lymphocytes, leukocytes and platelets onto a solid-phase support for use in solid-phase immunoassays through use of a drying solution and an article for use in immunoassays prepared by such method. The method comprises immobilizing a monolayer of cells onto the solid-phase support by non-covalent binding. This is accomplished by staining the solid-phase support with an organic dye having a net positive charge which permits non-covalent binding of the cells which carry a net negative charge to the solid-phase support. These cells are dried or fixed to the solid-phase support by addition of a drying solution which comprises an aqueous solution of a monosaccharide, disaccharide, trisaccharide or cyclitol and a salt. The preferred monosaccharide is D-(31 )glucose and the preferred salt is sodium chloride. The preferred drying solution comprises a 1.

4963478, Oct 16, 1990

Jul 5, 1988

7/215041

Lyle T. Sinor - Roswell GA
Ralph A. Eatz - Roswell GA
Darryl L. Stone - Roswell GA
Fred V. Plapp - Overland Park KS

Immucor, Inc. - Norcross GA

G01N 33543
G01N 33554

435 7

An article for performing solid-phase immunological assays and method for performing assays utilizing the article of the invention. A solid-phase support suitable for immunological assays is stained with an organic dye that has a net-positive charge and a hydrophobic aromatic ring structure. An immunologically reactive component having a net-negative charge is immobilized by the dye to the support through non-covalent interactions. The immunologically reactive component is tightly bound by the organic dye. This is advantageous when performing immunological assays utilizing automated washing instruments. A sample of a biological fluid is added to the article having the immunologically reactive component bound thereto to detect the presence of antigens or antibodies in the biological fluid specific for the bound immunological component. The presence of the antigen-antibody complex may be determined by any known method.