Ryan Duggan resumes & CV records
Resumes
Maintenance Specialist
Work:
Self Implo Ed
Maintenance Specialist
Maintenance Specialist
Ryan Duggan
Work:
Cambria Hotel Fort Mill
Ryan T Duggan, 42Crestwood, IL
Ryan Duggan Phones & Addresses
Midlothian, IL
Saint Charles, MO
Wickliffe, KY
Mentions for Ryan T Duggan
Publications & IP owners
Us Patents
Oligonucleotide-Mediated Quantitative Multiplexed Immunoassays
US Patent:
2017026, Sep 21, 2017
Filed:
May 26, 2017
Appl. No.:
15/606045
Inventors:
- Chicago IL, US
Ryan Duggan - Chicago IL, US
Stephen Kron - Chicago IL, US
Ryan Duggan - Chicago IL, US
Stephen Kron - Chicago IL, US
International Classification:
C12Q 1/68
Abstract:
Methods and compositions for quantitative immunoassays are provided, in which ligand-conjugated probes are used to label samples and ligand-surfaced microspheres are used as quantitative reference standards. Certain embodiments provide a method of quantitative flow cytometry where ligands are oligonucleotides, and a sample comprising one or more cells is contacted with a hybridized antibody::fluorophore labeled targeting construct to label the cells, and the labeled cells are analyzed. In some embodiments, a population of quantitative labeled oligospheres labeled with the same fluorescent label as the cells is analyzed using the flow cytometer and used to create a quantitative standard curve of cytometer intensity versus molecules fluorescent label per oligosphere event. A standard curve trendline is established and used to determine the molecules of fluorescent label per cellular event for the antigen-positive cell populations. Based on molecules of fluorescent label per cell, the amount of Antibody Binding per Cell (ABC) is quantified.
Oligonucleotide-Mediated Quantitative Multiplexed Immunoassays
US Patent:
2015034, Dec 3, 2015
Filed:
Jun 14, 2013
Appl. No.:
14/408065
Inventors:
- Chicago IL, US
Ryan Duggan - Chicago IL, US
Stephen Kron - Chicago IL, US
Ryan Duggan - Chicago IL, US
Stephen Kron - Chicago IL, US
International Classification:
C12Q 1/68
G01N 21/64
G01N 33/569
G01N 33/543
G01N 15/14
G01N 21/64
G01N 33/569
G01N 33/543
G01N 15/14
Abstract:
Methods and compositions for quantitative immunoassays are provided, in which ligand-conjugated probes are used to label samples and ligand-surfaced microspheres are used as quantitative reference standards. Certain embodiments provide a method of quantitative flow cytometry where ligands are oligonucleotides, and a sample comprising one or more cells is contacted with a hybridized antibody::fluorophore labeled targeting construct to label the cells, and the labeled cells are analyzed. In some embodiments, a population of quantitative labeled oligospheres labeled with the same fluorescent label as the cells is analyzed using the flow cytometer and used to create a quantitative standard curve of cytometer intensity versus molecules fluorescent label per oligosphere event. A standard curve trendline is established and used to determine the molecules of fluorescent label per cellular event for the antigen-positive cell populations. Based on molecules of fluorescent label per cell, the amount of Antibody Binding per Cell (ABC) is quantified.
NOTICE: You may not use BackgroundCheck or the information it provides to make decisions about employment, credit, housing or any other purpose that would require Fair Credit Reporting Act (FCRA) compliance. BackgroundCheck is not a Consumer Reporting Agency (CRA) as defined by the FCRA and does not provide consumer reports.