Inventors:
John Limanto - Metuchen NJ, US
Gregory Beutner - Green Brook NJ, US
Brendan Grau - Warrington PA, US
Jacob Janey - New York NY, US
Artis Klapars - Edison NJ, US
Eric Ashley - Fanwood NJ, US
Hallena R. Strotman - Monmouth Junction NJ, US
Matthew D. Truppo - Ocean Township NJ, US
Gregory Hughes - Scotch Plains NJ, US
Fabien L. Cabirol - Dusseldorf, DE
Anupam Gohel - Singapore, SG
Steven J. Collier - Singapore, SG
Jack Liang - San Mateo CA, US
Marissa Mock - Moorpark CA, US
Emily Mundorff - Poughkeepsie NY, US
Scott Novick - Palo Alto CA, US
Derek Smith - Singapore, SG
Assignee:
CODEXIS, INC. - Redwood City CA
International Classification:
C12N 9/10
US Classification:
435128, 536 232, 435193, 4353201, 4352523, 43525233, 43525235, 43525411, 4352542, 43525421, 43525423, 435348, 435325, 435358, 435365, 435352, 435366, 435419
Abstract:
The disclosure provides transaminase polypeptides capable of converting the substrate, 2-(3,4-dimethoxyphenethoxy)cyclohexanone to the trans diastereomer product (1R,2R)-2-(3,4-dimethoxyphenethoxy)cyclohexanamine in at least a 2:1 diastereomeric ratio relative to the cis diastereomer (1R,2S)-2-(3,4-dimethoxyphenethoxy)cyclohexanamine. The disclosure also provides polynucleotides, vectors, host cells, and methods of making and using the transaminase polypeptides in processes for preparing (1R,2R)-2-(3,4-dimethoxyphenethoxy)cyclohexanamine and its analogs, which can product compounds can be further used to prepare the aminocyclohexylether compound, (3R)-1-[(1R,2R)-2-[2-(3,4-dimethoxyphenyl)ethoxy]cyclohexyl]pyrrolidin-3-ol, which is an ion channel blocker.
