Stephen M Hewitt Deceased4201 Sideburn Rd, Fairfax, VA 22030

6951761, Oct 4, 2005

Aug 30, 2002

10/233193

Robert A. Star - Bethesda MD, US
Takehiko Miyaji - Rockville MD, US
Stephen M. Hewitt - Potomac MD, US
Lance A. Liotta - Bethesda MD, US

The United States of America as represented by the Department of Health and Human Services - Washington DC

G01N001/36
G01N001/00
G01N001/30
A01N001/02
C12Q001/00

436174, 436 8, 436 63, 435 11, 435 4, 435 5, 435 6, 435 71, 435 405, 435 911, 4353071, 435810

This disclosure relates to CryoArrays, which permit the analysis of samples (such as protein, nucleic acid, virus, or cell samples) in arrays that are prepared at low temperatures. Because CryoArrays are constructed as a block of substantially columnar samples, the block can be sliced to provide a plurality of identical or substantially identical individual arrays. The individual arrays can be used for parallel analysis of the same array feature set, for instance with different probes or under different conditions. Also provided are methods of making CryoArrays, devices for making CryoArrays, and kits.

6969615, Nov 29, 2005

Nov 20, 2001

10/432423

Vladimir Knezevic - Gaithersburg MD, US
Michael R. Emmert-Buck - Silver Spring MD, US
Galina Baibakova - Bethesda MD, US
Stephen M. Hewitt - Potomac MD, US
Capre Denise Mitchell - Winston Salem NC, US
Kevin Gardner - Montgomery Village MD, US

20/20 GeneSystems, Inc. - Rockville MD
The United States of America as represented by the Department of Health and Human Services - Washington MD

G01N033/543

436518, 422 681, 435 6, 435 71, 435 79, 435174, 4352871, 436524, 436528, 436530, 436531, 436503

The present disclosure is directed to devices, arrays, kits and methods for detecting biomolecules in a tissue section (such as a fresh or archival sample, tissue microarray, or cells harvested by an LCM procedure) or other substantially two-dimensional sample (such as an electrophoretic gel or cDNA microarray) by creating “carbon copies” of the biomolecules eluted from the sample and visualizing the biomolecules on the copies using one or more detector molecules (e. g. , antibodies or DNA probes) having specific affinity for the biomolecules of interest. Specific methods are provided for identifying the pattern of biomolecules (e. g. , proteins and nucleic acids) in the samples. Other specific methods are provided for the identification and analysis of proteins and other biological molecules produced by cells and/or tissue, especially human cells and/or tissue. The disclosure also provides a plurality of differentially prepared and/or processed membranes that can be used in described methods, and which permit the identification and analysis of biomolecules.

7854899, Dec 21, 2010

Aug 26, 2004

10/928656

Stephen M. Hewitt - Potomac MD, US

The United States of America as represented by the Secretary of Health and Human Services - Rockville MD

B01L 9/00

422104, 422102, 422 99

A method for preparing a microarray that includes placing at least one template over a first surface of the recipient block, wherein the template defines an array of openings and the recipient block has a plurality of receptacle holes, such that the array of openings are aligned with the plurality of receptacle holes. A needle or punch that contains a sample is inserted through the openings of the template. The sample then is inserted into the receptacle hole in the recipient block. A device is also disclosed that includes a platform defining (i) a first surface, and (ii) a first region configured to retain at least one recipient block; and a raised template defining an array of openings, secured to the first surface of the platform and positioned above the first region configured to retain the recipient block.

2011030, Dec 15, 2011

Jan 13, 2010

13/144474

Stephen M. Hewitt - Potomac MD, US
Joon-Yong Chung - Rockville MD, US

UNITED STATES DEPARTMENT OF HEALTH AND HUMAN SERVICES - Rockville MD

C40B 30/04
C12Q 1/34
G01N 21/64
H01J 49/26
C12Q 1/02
C40B 40/00
G01N 33/559
G01N 27/447
C12Q 1/48
C40B 30/00

506 9, 435 15, 435 18, 435 71, 506 7, 435 29, 506 13, 204456, 250282

Compositions, methods and kits are described for identifying biomolecules (e.g., proteins and nucleic acids) expressed in a biological sample that are associated with the presence, development, or progression of a disease (such as cancer), or more generally determination of the etiology or risk factors associated with a disease. Sample types analyzed by the disclosed methods include but are not limited to archival tissue blocks that have been preserved in a fixative, tissue biopsy samples, tissue microarrays, and so forth. The methods disclosed herein correlate expression profiles of biomolecules with various disease types, and allow for the determination of relative survival rates; in some embodiments, the methods permit determination of survival rates for a subject with cancer. In other embodiments, the disclosure relates to methods for evaluating therapeutic regimes for the treatment, such as treatment of cancer.