Steven E Fischer, 39Fremont, CA

6359275, Mar 19, 2002

Jul 14, 1999

09/352467

James L. Bertsch - Palo Alto CA
Steven M. Fischer - Hayward CA
Julie B. Riccomini - Saratoga CA

Agilent Technologies, Inc. - Palo Alto CA

H01J 4904

250281, 250288, 250282, 250423 R

A conduit for conducting ions from a high pressure ion source to a mass analyzer in mass spectrometry apparatus includes a capillary tube in which the lumenal surface of the bore near at least one end is a surface of an electrically conductive material. In one embodiment the conduit is constructed of a dielectric material and has an electrically conductive coating on an end portion of the lumenal surface.

6406633, Jun 18, 2002

Jul 6, 2000

09/611148

Steven M. Fischer - Hayward CA
Glen F. Ingle - Sunnyvale CA

Agilent Technologies, Inc. - Palo Alto CA

B01D 1508

210659, 210 85, 210143, 2101982, 436161, 73 6157

A fraction collection system for liquid chromatography includes a conduit, which directs a sample eluent from an LC column through a flow splitter to a destructive analytical detector such as a mass spectrometer to analyze sample components, and to a fraction collector. A nondestructive detector such as a photodetector is positioned near the fraction collector to detect arrival of sample components. Optionally, a nondestructive detector such as a photodetector is situated upstream from the splitter, to detect passing sample components. Time intervals between detection of a calibrant or a sample component at any two of the detectors provide for automatable identification of selected sample components at the sample collector.

6417511, Jul 9, 2002

Jul 17, 2000

09/617877

Steven M. Fischer - Hayward CA

Agilent Technologies, Inc. - Palo Alto CA

H01J 4900

250292, 250396 R

A ring pole ion guide apparatus and method provide the focusing and confinement advantages of conventional multipoles and the axial field of a conventional DC ring guide all in one device. The ring pole apparatus comprises a ring stack portion and a multipole portion, wherein the ring stack portion essentially overlaps the multipole portion inside and outside along a central axis. The ring pole apparatus can be used in a mass spectrometer system to guide ions from the ion source to the mass spectrometer or between mass spectrometer stages, or to dissociate ions into daughter ions in an ion dissociation system. A single ring pole ion guide can span a plurality of pressure transition stages with several of the rings acting as pressure partitions.

6486469, Nov 26, 2002

Oct 29, 1999

09/429063

Steven M. Fischer - Hayward CA
Robert K. Crawford - Palo Alto CA

Agilent Technologies, Inc. - Palo Alto CA

B01D 5944

250288, 250281, 250290, 250293

For delivery of ions from a higher pressure ion source to a mass analyzer operating at high vacuum, high pass ion filtration is effected within a dielectric capillary interface between the higher pressure ionization chamber and the lower pressure environment of a mass analyzer, by application of electrical potentials to end electrodes and to at least one electrode associated with the dielectric capillary between the ends, to create an end-to-end electric field generally opposing gas flow-assisted movement of ions from the upstream end to the downstream end, and to create a steeper voltage gradient along an upstream portion than along a downstream portion of the capillary. The voltage gradient along the steeper upstream portion of the capillary is sufficiently steep to cause ions having drift velocities below a lower limit to stall within the capillary. The respective potentials may be adjusted to increase the steepness of the upstream voltage gradient to increase the drift velocity lower limit.

6583407, Jun 24, 2003

Aug 23, 2000

09/645050

Steven M. Fischer - Hayward CA

Agilent Technologies, Inc. - Palo Alto CA

B01D 5944

250288, 250281, 250287, 250282

The present invention relates to a method for selectively delivering ions to a mass analyzer operating in a vacuum region. The method involves providing a dielectric conduit having an axial bore originating in an inlet opening that communicates with an ion source and terminating in an exit opening disposed within the vacuum region. A gas stream comprising an ion having a polarity and traveling at a drift velocity is flowed through the bore from the inlet opening toward the exit opening. A motion component is altered in a manner that does not substantially depend on the polarity of the ion to effect a change in the drift velocity of the ion. The invention also provides an apparatus to carry out the method.

6613523, Sep 2, 2003

Jun 29, 2001

09/896299

Steven M. Fischer - Hayward CA

Agilent Technologies, Inc. - Palo Alto CA

C12Q 168

435 6, 435 912, 435 77, 435 78, 536 2433, 536 253, 536 27

The present invention provides novel systems for sequencing nucleic acid molecules using dNTPs that are 3â end labeled with cleavable tags that block further extension and uniquely identify the bases to which they are attached. Removal of the tags liberates the 3â ends of the extension products for further extension. In related embodiments, oligonucleotides containing sequence-related cleavable tags are employed in a ligation reaction to determine the sequence of a particular DNA sample.

6646256, Nov 11, 2003

Dec 18, 2001

10/023140

Darrell L. Gourley - San Francisco CA
Steven M. Fischer - Hayward CA

Agilent Technologies, Inc. - Palo Alto CA

H01V 4928

250288, 250423 R, 315248

Apparatus and methods are disclosed for an ion source that comprises a flow-through ultraviolet lamp for photoionization. One embodiment of the apparatus comprises a tubular outer element and a tubular inner element. The inner element is disposed within the outer element to provide a space between the inner and outer elements. The inner element is open at its ends to provide a pathway through that element. A gas discharge between the elements produces ultraviolet radiation, which transmits through the wall of the inner element into the region within the inner element and ionizes molecules that flow through the region. The ion source can be employed in conjunction with a mass analyzer for mass spectrometry. In the method for ionizing molecules in an ion source, vaporized molecules are flowed through the region within the inner element, the region being surrounded by UV radiation. The radiation ionizes vaporized molecules in the surrounded region.

6646257, Nov 11, 2003

Sep 18, 2002

10/245987

Steven M. Fischer - Hayward CA
Darrell L. Gourley - San Francisco CA
James L. Bertsch - Palo Alto CA

Agilent Technologies, Inc. - Palo Alto CA

B01D 5944

250288, 250281, 250282

The present invention provides an apparatus and method for use with a mass spectrometer. The multimode ionization source of the present invention provides one or more atmospheric pressure ionization sources (i. e. electrospray, atmospheric pressure chemical ionization and/or atmospheric pressure photoionization) for ionizing molecules. A method of producing ions using the multimode ionization source is also disclosed. The apparatus and method provide the advantages of the combined ion sources without the inherent disadvantages of the individual sources.