Tommie H Lincecum2807 Sombrosa St, Carlsbad, CA 92009

2004020, Oct 14, 2004

Sep 20, 2002

10/251648

Susan Martinis - Houston TX, US
James Briggs - Katy TX, US
Richard Mursinna - Houston TX, US
Keun Lee - Houston TX, US
Tommie Lincecum - Houston TX, US
Caleb Davis - Houston TX, US
Meng Cui - Houston TX, US
Amy Williams - Houston TX, US
Joshua Speidel - New York NY, US
Yuxin Zhai - Houston TX, US

C07K014/47
C07H021/04
C12P021/06

435/069100, 435/455000, 435/320100, 435/325000, 530/350000, 536/023500

A method and composition for tRNA synthetases that activate and aminoacylate nonstandard and noncognate amino acids to tRNA adaptor molecules is described that can be used to generate custom designed protein products for uses in medicinal, therapeutic, diagnostic, biotechnology, engineering, and spectroscopy applications. Some tRNA synthetases naturally misactivate and misaminoacylate noncognate amino acids. Many of these tRNA synthetases, including but not limited to leucyl-, isoleucyl-, and valyl-tRNA synthetases, have evolved proofreading and editing mechanisms to correct these mistakes. Inactivation of the enzyme's editing activity allows and facilitates production and accumulation of tRNAs that are misaminoacylated with nonstandard and noncognate amino acids. These misaminoacylated tRNAs can be used to introduce novel amino acids into proteins.

2008007, Mar 27, 2008

Mar 30, 2007

11/694605

Yuri Belosludtsev - The Woodlands TX, US
Mistu Reddy - Pearland TX, US
Anelia Kraltcheva - Houston TX, US
Susan Hardin - College Station TX, US
Tommie Lincecum - Houston TX, US
Hongyi Wang - Houston TX, US
Norha Deluge - Houston TX, US
Uma Nagaswamy - Houston TX, US
Benjamin Stevens - Houston TX, US
Kristi Kincaid - Houston TX, US

VISIGEN BIOTECHNOLOGIES, INC. - Houston TX

G01N 21/64

436172000

Support surfaces are disclosed that are designed to support molecules or molecular assemblies immobilized thereon so that the molecules or molecular assemblies can be observed in single molecule detections systems, where the support surfaces have reduced background and the fluorescent labels associated with the immobilized molecules or molecular assemblies have longer active lifetimes prior to permanent photo-bleaching or deactivation and have improve fluorescence properties and where the surfaces have more uniform fluorescent properties.

2008009, Apr 17, 2008

Jul 20, 2007

11/781160

Hongyi Wang - Houston TX, US
Xiaolian Gao - Houston TX, US
Peilin Yu - Houston TX, US
Mitsu Reddy - Pearland TX, US
Susan Hardin - College Station TX, US
Tommie Lincecum - Houston TX, US
Amy Williams - Houston TX, US
Norha Deluge - Houston TX, US
Yuri Belosludtsev - The weedlands TX, US
Steven Menchen - Fremont CA, US
Joe Lam - Castro Valley CA, US

VISIGEN BIOTECHNOLOGIES, INC. - Houston TX
APPLIED BIOSYSTEMS - Foster City CA

C07H 21/04

536026600

Modified nucleotides are disclosed for use in single molecule sequencing, methods for making the modified nucleotides and method for using the modified nucleotides. Linkers for making the modified nucleotide are also disclosed.

2013004, Feb 14, 2013

Aug 10, 2012

13/572488

Peter VANDER HORN - Encinitas CA, US
Theo NIKIFOROV - Carlsbad CA, US
Guobin LUO - Oceanside CA, US
Mindy LANDES - Carlsbad CA, US
Daniel MAZUR - San Diego CA, US
Eileen TOZER - San Diego CA, US
Tommie LINCECUM - Houston TX, US

LIFE TECHNOLOGIES CORPORATION - Carlsbad CA

C12N 9/12

435194

The present disclosure provides compositions, methods, kits, systems and apparatus that are useful for nucleic acid polymerization. In particular, modified polymerases and biologically active fragment thereof are provided that allow for nucleic acid amplification. In one aspect, the disclosure relates to modified polymerases useful for nucleic acid sequencing, genotyping, copy number variation analysis, paired-end sequencing and other forms of genetic analysis. In some aspects, the disclosure relates to modified polymerases useful for the generation of nucleic acid libraries or nucleic acid templates for use in various downstream processes. In some aspects, the disclosure relates to the identification of homologous amino acid mutations that can be transferred across classes or families of polymerases to provide novel polymerases with altered catalytic properties. In some aspects, the disclosure provides modified polymerases having enhanced catalytic properties as compared to a reference polymerase.

2022030, Sep 29, 2022

Apr 20, 2022

17/725369

- Carlsbad CA, US
Eileen Tozer - San Diego CA, US
Sihong Chen - Vista CA, US
Peter Vander Horn - Encinitas CA, US
Tommie Lincecum - Carlsbad CA, US

Life Technologies Corporation - Carlsbad CA

C12Q 1/686
C12Q 1/6869
C12N 9/12

The present disclosure provides compositions, methods, kits, systems and apparatus that are useful for nucleic acid polymerization. In particular, modified polymerases and biologically active fragments thereof, such as modified Taq polymerases, are provided that allow for improved nucleic acid amplification. In some aspects, the disclosure provides modified polymerases having improved thermostability, accuracy, processivity and/or read length as compared to a referenceTaq polymerase. In some aspects, the disclosure relates to modified polymerases or biologically active fragments thereof, useful for amplification methods, and in practically illustrative embodiments, emulsion PCR.

2020038, Dec 10, 2020

Aug 14, 2020

16/947742

- Carlsbad CA, US
Eileen TOZER - San Diego CA, US
Sihong CHEN - Vista CA, US
Peter VANDER HORN - Encinitas CA, US
Tommie LINCECUM - Carlsbad CA, US

C12Q 1/686
C12Q 1/6869
C12N 9/12

The present disclosure provides compositions, methods, kits, systems and apparatus that are useful for nucleic acid polymerization. In particular, modified polymerases and biologically active fragments thereof, such as modified Taq polymerases, are provided that allow for improved nucleic acid amplification. In some aspects, the disclosure provides modified polymerases having improved thermostability, accuracy, processivity and/or read length as compared to a referenceTaq polymerase. In some aspects, the disclosure relates to modified polymerases or biologically active fragments thereof, useful for amplification methods, and in practically illustrative embodiments, emulsion PCR.

2020027, Sep 3, 2020

Apr 16, 2020

16/850629

- Carlsbad CA, US
Theo NIKIFOROV - Carlsbad CA, US
Guobin LUO - Oceanside CA, US
Mindy LANDES - Carlsbad CA, US
Daniel MAZUR - San Diego CA, US
Eileen TOZER - San Diego CA, US
Tommie LINCECUM - Carlsbad CA, US

C12N 9/12
C12Q 1/6844
C12Q 1/686

The present disclosure provides compositions, methods, kits, systems and apparatus that are useful for nucleic acid polymerization. In particular, modified polymerases and biologically active fragment thereof are provided that allow for nucleic acid amplification. In one aspect, the disclosure relates to modified polymerases useful for nucleic acid sequencing, genotyping, copy number variation analysis, paired-end sequencing and other forms of genetic analysis. In some aspects, the disclosure relates to modified polymerases useful for the generation of nucleic acid libraries or nucleic acid templates for use in various downstream processes. In some aspects, the disclosure relates to the identification of homologous amino acid mutations that can be transferred across classes or families of polymerases to provide novel polymerases with altered catalytic properties. In some aspects, the disclosure provides modified polymerases having enhanced catalytic properties as compared to a reference polymerase.

2019006, Feb 28, 2019

Sep 6, 2018

16/123166

- Carlsbad CA, US
Daniel MAZUR - San Diego CA, US
Xinzhan PENG - Carlsbad CA, US
Tommie Lloyd LINCECUM - Houston TX, US
Yuri BELOSLUDTSEV - Tucson AZ, US
Howard REESE - Poway CA, US
Dmitriy GREMYACHINSKIY - San Francisco CA, US
Roman ROZHKOV - Redwood City CA, US
John MAURO - Eugene OR, US
Joseph BEECHEM - Eugene OR, US
Eric TULSKY - Berkeley CA, US
Imad NAASANI - Manchester, GB
Kari HALEY - Portland OR, US
Joseph TREADWAY - Eugene OR, US

C12Q 1/6869
C12N 9/12
G01N 33/58
G01N 21/64
C07H 19/20
C12Q 1/6818
C12N 9/96

Disclosed herein are conjugates comprising a biomolecule linked to a label that have biological activity and are useful in a wide variety of biological applications. For example, provided herein are polymerase-nanoparticle conjugates including a polymerase linked to a nanoparticle, wherein the conjugate has polymerase activity. Such conjugates can exhibit reduced aggregation and improved stochiometries wherein the average biomolecule:nanoparticle ratio approaches or equals 1:1. Also disclosed herein are improved methods for preparing such conjugates, and methods and systems for using such conjugates in biological applications such as nucleotide incorporation, primer extension and single molecule sequencing.