Viola Vogel11520 20Th Ave NE, Seattle, WA 98125

2003018, Oct 2, 2003

Aug 26, 2002

10/229820

Viola Vogel - Seattle WA, US
Andre Krammer - Seattle WA, US
Klaus Schulten - Urbana IL, US
Barry Isralewitz - Urbana IL, US
Hui Lu - Urbana IL, US

G01N033/53
C12M001/34
G06F019/00
G01N033/48
G01N033/50

435/007100, 435/287200, 702/019000

The present disclosure provides force-regulated molecular switches and methods for controlling binding and release of a ligand (cell, protein or other polymer, or small molecule) to the switch-containing device by the application, release or modulation of force (physical tension or an electrical or magnetic field as specifically exemplified herein). The FRMR switch technology can be applied to vectorial pumps, molecule-specific sponges, calorimetric cell motility assays, electronically addressable biorecognition arrays, cell sorting devices, tissue engineering scaffolds, calorimetric affinity assays, diagnostics and therapeutics.

2003021, Nov 20, 2003

Dec 5, 2002

10/314453

Prabha Nair - Trivandrum, IN
Buddy Ratner - Seattle WA, US
Viola Vogel - Seattle WA, US
Robert Nerem - Atlanta GA, US

C12N005/00
C12N005/02

435/395000

In one aspect the present invention provides biodegradable, porous structures that each include a structural framework, wherein the structural framework includes: (a) a solidified mixture of polymer molecules and amino acid molecules, wherein at least some of the amino acid molecules are linked to other amino acid molecules within the framework; and (b) a multiplicity of interconnected spaces defined by the structural framework. In some embodiments, the porous structures of the invention are adapted to physically support the growth of living cells in vitro or in vivo, and can be used to grow living tissue and/or living organs. The present invention also provide methods for making the biodegradable, porous structures of the invention.

2004006, Apr 8, 2004

Jun 27, 2003

10/607834

Viola Vogel - Seattle WA, US
Wendy Thomas - Seattle WA, US
Manu Forero - Seattle WA, US
Evgeni Sokurenko - Seattle WA, US

C12Q001/68
G01N033/554
G01N033/569

435/007320

Methods, compositions and devices are provided based on changing the binding strength of an adhesion molecule to a ligand by changing the force exerted on the bound complex between adhesion molecule and ligand, for example by changing the shear stress acting on the complex. The adhesion molecules and their ligands of this invention bind more tightly when a force-activated bond stress, such as shear force, applied to the adhesion molecules is increased, and bond less tightly when the stress is decreased. The adhesion molecules can be isolated from their sources in nature or can remain attached to their natural sources. They can be engineered, e.g., by altering their amino acid sequences or by binding to antibodies or other particles, to alter their binding properties. They can be attached to a wide range of substrates including particles and device surfaces to form adhesive systems which are capable of sticking to other particles and/or device surfaces to which ligands for the adhesion molecules have been attached. The adhesion molecules and ligands described herein can be used to control binding and release of components of an adhesive system by increasing or decreasing the force-activated bond stresses applied to the adhesion molecules.

2009032, Dec 31, 2009

Jan 15, 2009

12/354693

Viola Vogel - Seattle WA, US
Wendy Thomas - Seattle WA, US
Manu Forero - Seattle WA, US
Evgeni Sokurenko - Seattle WA, US

UNIVERSITY OF WASHINGTON - Seattle WA

C12N 11/00
C07K 14/00
C07K 16/00
C07K 14/42

435174, 530402, 530395, 5303911, 530370

Methods, compositions and devices are provided based on changing the binding strength of an adhesion molecule to a ligand by changing the force exerted on the bound complex between adhesion molecule and ligand, for example by changing the shear stress acting on the complex. The adhesion molecules and their ligands of this invention bind more tightly when a force-activated bond stress, such as shear force, applied to the adhesion molecules is increased, and bond less tightly when the stress is decreased. The adhesion molecules can be isolated from their sources in nature or can remain attached to their natural sources. They can be engineered, e.g., by altering their amino acid sequences or by binding to antibodies or other particles, to alter their binding properties. They can be attached to a wide range of substrates including particles and device surfaces to form adhesive systems which are capable of sticking to other particles and/or device surfaces to which ligands for the adhesion molecules have been attached. The adhesion molecules and ligands described herein can be used to control binding and release of components of an adhesive system by increasing or decreasing the force-activated bond stresses applied to the adhesion molecules.