Haifeng E Chen, 6547 Saint James Pl, Piedmont, CA 94611

7115391, Oct 3, 2006

Sep 29, 2000

10/089394

Haifeng Chen - Castro Valley CA, US
Gary Kurtzman - Bryn Mawr PA, US

Genovo, Inc. - Seattle WA

C12P 21/06
C12N 15/63
C12N 15/64
A61K 39/12
A01N 63/00

435 691, 435455, 435456, 4353201, 435 9141, 435 9142, 424 932, 424 9321, 4241991

This invention relates to novel adenoviruses useful in the production of high titers of recombinant adeno-associated virus (rAAV) comprising a foreign DNA insert and methods of making these adenoviruses. The adenovirus comprises the AAV rep gene in which the p5 promoter is replaced by a minimal promoter or by no promoter. The invention also provides methods of producing high levels of rAAV as a substantially homogenous preparation and composition of rAAV.

2005001, Jan 27, 2005

Aug 17, 2004

10/920647

Siyamak Rasty - Wilmington DE, US
Matthew Gonda - Vestavia Hills AL, US
Haifeng Chen - Media PA, US

Genovo, Inc. - Seattle WA

C12N015/86

435456000

This invention relates to novel nonmammalian carrier vectors and viruses useful in the production of high titers of recombinant viruses which may contain foreign DNA inserts or which may be point-mutated or deleted viruses, and methods of producing those viruses. The nonmammalian carrier vector (“carrier vector”) is a chimeric vector which includes those portions of a nonmammalian virus backbone which allow replication in a nonmammalian host cell. The carrier vector includes various nucleic acid cassettes, which may include an embedded recombinant viral genome containing a desired transgene, components necessary for production of a replication-defective recombinant virus containing the transgene, and domains that permit the carrier vector to bind to mammalian cells. The invention also provides methods of producing high concentrations of recombinant virus as a substantially homogeneous. preparation, compositions to produce the recombinant virus, and novel recombinant viruses.

2007006, Mar 22, 2007

Aug 25, 2006

11/509855

Haifeng Chen - Media PA, US
Gary Kurtzman - Bryn Mawr PA, US

A61K 48/00
C12N 15/861

424093200, 435456000, 977802000

This invention relates to novel adenoviruses useful in the production of high titers of recombinant adeno-associated virus (rAAV) comprising a foreign DNA inert and methods of making these adenoviruses. The adenovirus comprises the AAV rep gene in which the p5 promoter of rep is replaced by a minimal promoter or by no promoter. The invention also provides methods of producing high levels of rAAV as a substantially homogeneous preparation and compositions of rAAV.

2009019, Jul 30, 2009

Jan 22, 2007

12/161202

Jude Samulski - Chapel Hill NC, US
Haifeng Chen - Castro Valley CA, US

ASKLEPIOS BIOPHARMACEUTICAL, INC. - Chapel Hill NC

C12P 19/34
C12N 5/06

435 914, 435325

A method of producing a packaged parvovirus vector, the method comprising: (a) providing an insect cell; (b) introducing into the insect cell one or more vectors comprising nucleotide sequences encoding: (i) a transgene flanked by TRs; and (ii) baculovirus packaging functions comprising Rep components and Cap components sufficient to result in packaging of infective parvovirus particles, wherein VP1 is supplemented relative to VP2 and VP3 sufficient to increase the production of infectious viral particles; and (c) introducing into the cell a nucleic acid encoding baculovirus helper functions for expression in the insect cell; (d) culturing the cell under conditions sufficient to produce the infectious packaged parvovirus vector.

2009020, Aug 13, 2009

Aug 24, 2007

12/297958

Haifeng Chen - Castro Valley CA, US

Virovek, Inc. - Hayward CA

C12P 21/06
C12P 19/34
C12N 7/00
C12N 5/10
C12N 15/63
C07H 21/04

435 691, 435 911, 4352351, 435348, 4353201, 536 235

The present teachings disclose nucleic acid cassettes for expressing in an insect cell a plurality of polypeptides encoded by a gene comprising overlapping open reading frames (ORFs). A cassette comprises, in 5′ to 3′ order, a) a first insect cell-operable promoter, b) a 5′ portion of a gene comprising a first ORF of the gene, c) an intron comprising a second insect cell-operable promoter, and d) a 3′ portion of the gene comprising at least one additional ORF. Vectors and insect cells comprising the cassettes are also disclosed, as well as methods for production of recombinant adeno-associated virus in insect cells using the cassettes.

6793926, Sep 21, 2004

May 25, 2000

09/578561

Siyamak Rasty - Wilmington DE
Matthew A. Gonda - Vestavia Hills AL
Haifeng Chen - Media PA

Genovo, Inc. - Seattle WA

A61K 3912

4241991, 4241841, 4242041, 4242331, 435 691, 4352351, 4353201, 435455, 435456, 435457, 514 44, 536 231, 536 2372

This invention relates to novel nonmammalian carrier vectors and viruses useful in the production of high titers of recombinant viruses which may contain foreign DNA inserts or which may be point-mutated or deleted viruses, and methods of producing those viruses. The nonmammalian carrier vector (âcarrier vectorâ) is a chimeric vector which includes those portions of a nonmammalian virus backbone which allow replication in a nonmammalian host cell. The carrier vector includes various nucleic acid cassettes, which may include an embedded recombinant viral genome containing a desired transgene, components necessary for production of a replication-defective recombinant virus containing the transgene, and domains that permit the carrier vector to bind to mammalian cells. The invention also provides methods of producing high concentrations of recombinant virus as a substantially homogeneous preparation, compositions to produce the recombinant virus, and novel recombinant viruses.

2020040, Dec 31, 2020

Jun 25, 2020

16/912084

- Hayward CA, US
Haifeng Chen - Hayward CA, US

C12N 7/00
C12N 15/86
A01K 67/033

The present disclosure relates to a heterologous recombinant baculovirus (rBV) expression system for the production of foreign heterologous proteins in insect cells. This system comprises a recombinant baculovirus backbone within a genome with a deletion in the cathepsin gene into which foreign gene cassettes can be integrated, and an insect cell that can be infected by the Δv-cath-rBV, and in which the foreign proteins and/or viral vectors or particles are expressed.

2019016, May 30, 2019

Nov 28, 2018

16/203055

- Hayward CA, US
Haifeng Chen - Piedmont CA, US

C12N 1/02
C12N 1/04
C12N 1/06
C12N 5/07

Methods of purifying a virus from a virus-infected cell lysate using three-phase partitioning (TPP) are disclosed. The methods comprise a first round of TPP, including mixing a cell lysate comprising a virus with ammonium sulfate and t-butanol, and separating the mixture, thereby forming a first aqueous phase, a first organic phase, and a first interphase. The first aqueous phase can comprise the virus, which can be subjected to a second round of TPP, resulting in a second aqueous phase, a second organic phase, and a second interphase. The second interphase can comprise highly purified virus. The methods can comprise subjecting a first aqueous phase to further purification by column chromatography or density gradient centrifugation. Purification of AAV, including AAV2, AAV5 and AAV6, from lysates of infected insect cell cultures is demonstrated. TPP-purified AAV particles infect at least as well as those prepared by standard methods.