Robert J Defranco, 78657 Walnut St UNIT 334, San Carlos, CA 94070

5462748, Oct 31, 1995

Jan 14, 1994

8/183104

David H. Lloyd - Daly City CA
Robert J. DeFranco - San Carlos CA
Charles S. Ladd - Union City CA

Applied Biosystems, Inc. - Foster City CA

A61K 910
A61K 916
C07K 106
C07K 110
B65D 326
B01J 1918

424484

A cartridge containing a dry polymer composition carrying trapped biopolymer subunits for use in synthesis of biopolymer chains provides a discrete unit for stepwise supply of subunits, such as protected amino acids, in an automated apparatus for biopolymer synthesis. The composition is swellable in organic solvent to release and supply the subunits to a growing biopolymer chain immobilized on a polymer support. An automated synthesizer uses the cartridges for sequential subunit supply and monitors conductivity of deprotection reagents to control cycling and timing of steps.

5298259, Mar 29, 1994

Nov 5, 1991

7/788322

David H. Lloyd - San Francisco CA
Robert J. DeFranco - San Carlos CA

Applied Biosystems, Inc. - Foster City CA

A61K 910
A61K 916
C07K 106
B65D 326

424486

A polymer composition having a biopolymer subunit entrapped in a swellable polymer matrix is described. The composition may be supplied in a disposable cartridge, for use in delivering a solution of the subunit in an automated biopolymer synthesis operation.

5665603, Sep 9, 1997

Jul 8, 1994

8/272496

Victoria L. Boyd - San Carlos CA
MeriLisa Bozzini - Burlingame CA
Robert J. DeFranco - San Carlos CA

The Perkin-Elmer Corporation - Foster City CA

A61K 3802

436 90

A method of forming a thiohydantoin from an N-protected amino acid is described. The method employs a phosphate compound selected from the group consisting of (R. sub. 1 O)(R. sub. 2 O)P(. dbd. O)X and (R. sub. 1 O)(R. sub. 2 O)P(. dbd. O)--O--P(. dbd. O)(OR. sub. 3)(OR. sub. 4) to form acylphosphate moieties from the carboxyl groups of internal aspartic acid and glutamic acid residues and an acylphosphate moiety at a C-terminal carboxyl. The later acylphosphate, unlike the internal acylphosphates, spontaneously cyclizes to an oxazolone, which is less reactive with nucleophilic reagents. R. sub. 1 and R. sub. 2 are each alkyl, aryl, or alkaryl groups which are the same or different and which may be covalently linked to each other; R. sub. 3 and R. sub. 4 are each alkyl, aryl, or alkaryl groups which are the same or different and which may be covalently linked to each other; and X is a leaving group, such as chlorine or bromine, which is substantially unreactive towards thiohydantoins. The acylphosphate and oxazolone moieties are then reacted with a thiocyanate reagent under conditions effective to convert the internal acylphosphates to amides and the terminal oxazolone to thiohydantoin, thereby permitting selective C-terminal thiodantionation of aspartic acid- and/or glutamic acid-containing proteins.

3975399, Aug 17, 1976

Aug 6, 1974

5/495199

Robert Jay DeFranco - Wilmington DE
Richard M. Scribner - Wilmington DE

E. I. Du Pont de Nemours and Company - Wilmington DE

C07D20728
C07D20730

2603262

Certain 1,5-disubstituted 2-pyrrolidinones, and their ring unsaturated analogs, resemble natural prostaglandins. The compounds are easier to prepare than the prostaglandins since they have at least one fewer center of asymmetry and accordingly fewer isomers are produced. Exemplary is 7-[2-oxo-5-(3-hydroxy-1-oct-1-enyl)-1-pyrrolidinyl]heptanoic acid of the formula: ##SPC1##.